Anti-SMURF2 Monoclonal Antibody for WB, IHC-P, IP, ELISA - Q9HAU4

Core Product Specifications and Parameters

Biological Background: SMURF2 Function and Localization

• SMURF2 is an E3 ubiquitin-protein ligase that accepts ubiquitin from an E2 enzyme and directly transfers it to targeted substrates. Related references: PMID:11016919 PMID:38016474
• Interacts with SMAD7 to trigger TGF-beta receptor ubiquitin-dependent degradation, thereby down-regulating TGF-beta signaling. Related references: PMID:11163210 PMID:12717440 PMID:21791611
• Forms a stable complex with phosphorylated SMAD1, SMAD2, and SMAD3, and targets SMAD1 and SMAD2 for ubiquitination and proteasomal degradation. Related references: PMID:11016919 PMID:11158580 PMID:11389444
• Negatively regulates TGFB1-induced epithelial-mesenchymal transition and myofibroblast differentiation. Related references: PMID:30696809
• Acts as an activator of ferroptosis by mediating ubiquitination and degradation of GSTP1, thereby preventing detoxification of reactive aldehydes. Related references: PMID:38016474
• Positively regulates smoothened signaling pathway by mediating ubiquitination of PTCH1, leading to its lysosomal degradation.
• In case of filovirus infection, the viral matrix protein VP40 forms a complex with SMURF2 to facilitate virus budding.
• Localizes to the nucleus, cytoplasm, cell membrane, and membrane rafts; widely expressed across tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region conserved in human SMURF2, supporting cross-reactivity with mouse; validate in relevant sample systems.
• For western blotting, a band near 86 kDa is expected; consider using positive and negative controls to confirm specificity.
• In immunohistochemistry (IHC-P), antigen retrieval conditions may require optimization; test known positive tissues as controls.
• The antibody's suitability for immunoprecipitation (IP) and ELISA suggests recognition of both native and denatured forms; confirm with appropriate lysates or recombinant protein.
• As SMURF2 is widely expressed, select cell lines or tissues relevant to your pathway of interest (e.g., TGF-beta signaling, ferroptosis) and include loading controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to validated IVD raw materials, including monoclonal antibodies like this anti-SMURF2 antibody, optimized for ubiquitination and TGF-beta pathway studies. Beyond individual antibodies, we offer matched antibody pairs, bulk ancillary reagents, and sourcing support for rare targets, empowering your assay development from concept to clinic. Let CamelBio be your reliable sourcing partner for next-generation IVD solutions.