Anti-SMU1 Polyclonal Antibody for WB, ELISA - Q2TAY7

Core Product Specifications and Parameters

Biological Background: SMU1 Function and Localization

• SMU1, also known as BWD, SMU-1, or fSAP57, is a WD40 repeat-containing protein that acts as a component of the spliceosome, involved in pre-mRNA splicing (PubMed:28781166). Related references: PMID:28781166
• It regulates alternative splicing of specific pre-mRNAs, such as HSPG2, thereby influencing protein diversity (By similarity).
• SMU1 is required for the normal accumulation of the IK protein, indicating a role in protein stability or expression regulation (PubMed:24945353). Related references: PMID:24945353
• It plays a critical role in mitotic spindle assembly and normal progression through mitosis, highlighting its importance in cell division (By similarity).
• Subcellularly, SMU1 localizes to the cytoplasm, nucleus, and nuclear speckles, consistent with its splicing functions.
• During influenza A virus infection, SMU1, together with IK, is required for the normal splicing of viral NS1 pre-mRNA, which is essential for virus particle production.
• Post-translational modifications include acetylation, isopeptide bond formation, and ubiquitin conjugation, which may regulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein corresponding to the N-terminal region (amino acids 1-220) of human SMU1; therefore, the antibody is expected to recognize epitopes within this domain.
• For Western blot (WB), the predicted molecular weight of SMU1 is approximately 58 kDa; validate the band size in your specific sample system.
• Due to the polyclonal nature, batch-to-batch variability may exist; consider including appropriate controls and validating each new lot.
• When using this antibody for ELISA, ensure proper coating and detection conditions, and validate with known positive and negative samples.
• This antibody shows cross-reactivity with mouse and rat SMU1, which can be advantageous for studies across these species; however, confirm sequence similarity in the immunogen region.
• For studies involving viral infection or mitosis, consider using specific synchronized cell models or infection protocols to observe SMU1 dynamics.

CamelBio: Your One-Stop Sourcing Bridge

As a leading one-stop IVD raw-material sourcing partner, CamelBio offers validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to support your splicing, mitosis, and viral research. Whether you need this anti-SMU1 antibody or rare-target raw materials, our supply chain ensures consistent quality and efficient procurement. Partner with us to accelerate your diagnostic development from concept to clinic.