Anti-SMTN Polyclonal Antibody for WB, ELISA - P53814

Core Product Specifications and Parameters

Biological Background: Smoothelin Function and Localization

• Smoothelin (SMTN) is a structural protein of the cytoskeleton encoded by the SMTN gene. Alternative promoter usage and splicing generate multiple isoforms, including a long form that is specifically expressed in smooth muscle cells.
• The protein localizes to the cytoplasm and associates with the cytoskeleton, playing a role in the organization and contractile function of smooth muscle tissues.
• Tissue-specific expression: the long isoform is predominantly found in contractile (vascular and visceral) smooth muscle, while other isoforms may have different distribution patterns.
• Post-translational modifications include acetylation and phosphorylation, which may regulate smoothelin’s function and protein-protein interactions.
• Structural features such as a coiled-coil domain suggest its capacity for protein-protein interactions, potentially contributing to cytoskeletal assembly.
• The calculated molecular weight of the canonical isoform is approximately 99 kDa, consistent with experimental observations.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 65–325 of human SMTN (NP_001193946.1), a region likely conserved across isoforms and species; consider verifying detection of specific isoforms in your target sample.
• For Western blotting, optimize antibody dilution and blocking conditions using the appropriate positive control lysate (e.g., smooth muscle tissue from human, mouse, or rat) to achieve clear, specific bands.
• When setting up ELISA, test a range of coating antigen concentrations and antibody dilutions to establish a robust standard curve; include cross-reactivity controls for the species of interest.
• Given cross-reactivity with human, mouse, and rat, this antibody can be used for multispecies studies, but always validate in the relevant species and tissue context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of high-quality IVD raw materials, including validated polyclonal antibodies like this anti-SMTN antibody, supporting smooth muscle biology and cytoskeleton research. With a commitment to streamlined sourcing, we also supply antibody pairs, monoclonal antibodies, and bulk ancillary reagents tailored to your assay development needs.