Anti-SMPD3/NSMase2 Monoclonal Antibody for WB, IHC-P, ELISA - Q9NY59

Core Product Specifications and Parameters

Biological Background: SMPD3/NSMase2 Function and Localization

• SMPD3 encodes neutral sphingomyelinase 2 (nSMase2), a sphingomyelin phosphodiesterase that hydrolyzes sphingomyelin to ceramide and phosphocholine.
  Related references: PMID:10823942, PMID:14741383, PMID:15051724
• Ceramide serves as a lipid second messenger that independently regulates apoptosis and growth arrest.
• The enzyme binds anionic phospholipids such as phosphatidylserine and phosphatidic acid, which modulate its enzymatic activity and subcellular localization (By similarity).
• nSMase2 localizes primarily to the Golgi apparatus membrane and the cell membrane.
• It is predominantly expressed in the brain, indicating a specialized role in neuronal ceramide signaling.
• Under inflammatory conditions, nSMase2 may mediate transcriptional upregulation of iNOS via NF-κB activation (By similarity).
• Post-translational modifications include phosphorylation and palmitoylation; the protein is implicated in cell cycle control and developmental processes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C‑terminal region (amino acids 461–655) of human SMPD3/NSMase2. Consider that splice variants lacking this epitope may not be detected.
• For western blotting, the expected band is approximately 71 kDa; include appropriate positive and negative controls and validate in the relevant sample system.
• For IHC‑P, optimal antigen retrieval conditions should be determined empirically. Brain tissue sections, where nSMase2 is highly expressed, may serve as a useful positive control.
• When using this antibody in ELISA, verify its performance as a capture or detection reagent in your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop platform for IVD raw material sourcing, delivering validated monoclonal antibodies like this SMPD3/NSMase2 antibody along with bulk ancillary reagents and rare target materials. Whether you need optimized antibody pairs, polyclonal alternatives, or technical consulting, our supply chain supports your sphingolipid metabolism and bone mineralization research projects from concept to clinic.