Anti-SMPD3 Rabbit Polyclonal Antibody for WB and ELISA - Q9NY59

Core Product Specifications and Parameters

Biological Background: SMPD3 Function and Localization

• SMPD3 encodes neutral sphingomyelinase 2 (nSMase2), which hydrolyzes sphingomyelin to generate the bioactive lipid ceramide and phosphocholine.
• Ceramide produced by SMPD3 mediates cellular apoptosis and growth arrest, and the enzyme can independently regulate these two processes.
• SMPD3 functions as a growth suppressor in confluent cells, thereby regulating the cell cycle.
• SMPD3 plays a critical role in postnatal development, contributing to bone and dentin mineralization. Related references: PMID:10823942 PMID:14741383 PMID:15051724
• SMPD3 also hydrolyzes sphingosylphosphocholine and binds anionic phospholipids such as phosphatidylserine and phosphatidic acid, which modulate its activity and subcellular localization.
• Subcellularly, SMPD3 localizes to the Golgi apparatus membrane and the cell membrane.
• SMPD3 undergoes alternative splicing to produce multiple transcript variants.
• Predominantly expressed in the brain, SMPD3 may be involved in IL-1β-induced JNK activation in hepatocytes and NF-κB-mediated regulation of NOS2/iNOS under inflammatory conditions (By similarity).

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 416-655) which may confer isoform specificity; validate antibody specificity using peptide blocking or knockdown controls for your target system.
• For Western blot, the expected band is at ~71 kDa. Optimize protein loading and blocking conditions, and use brain tissue lysates (human, mouse, or rat) as positive controls given the high brain expression.
• In ELISA applications, perform serial dilutions of the antibody to determine optimal working concentration; include recombinant SMPD3 protein as a positive control.
• Palmitoylation of SMPD3 (as indicated by UniProt) may influence migration or antibody binding; denaturing and reducing conditions should be maintained during sample preparation.
• Cross-reactivity with mouse and rat is reported; however, verify staining patterns against knockout or over-expression models to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material sourcing, linking diagnostic developers with validated antibodies, recombinant proteins, and ancillary reagents. For sphingolipid metabolism and apoptosis research, we provide optimized antibody pairs, bulk polyclonal and monoclonal antibodies, and support for rare target procurement—streamlining your transition from discovery to clinical assay development. Partner with CamelBio to secure high-quality reagents tailored to your diagnostic pipeline.