Anti-SMPD2 Rabbit Polyclonal Antibody for Western Blot and ELISA - O60906

Core Product Specifications and Parameters

Biological Background: SMPD2 Function and Localization

• SMPD2, also known as neutral sphingomyelinase (nSMase), Lyso-PAF-PLC, ISC1, and NSMASE1, is encoded by the SMPD2 gene in humans.
• It catalyzes the hydrolysis of sphingomyelin to ceramide and phosphocholine, and additionally hydrolyzes lyso-PAF, lyso-PC, and sphingosylphosphocholine.
  Related references: PMID:10608884, PMID:14741383
• The enzyme is localized at the cell membrane and contains transmembrane helices.
• SMPD2 requires magnesium ions for its catalytic activity and plays a key role in sphingolipid metabolism.
• It is a member of the neutral sphingomyelinase family, which participates in lipid signaling and cellular stress responses.
• The protein is annotated with keywords including hydrolase, lipid metabolism, metal-binding, sphingolipid metabolism, and transmembrane.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 150–250 of human SMPD2 (NP_003071.2), a region conserved in mouse and rat, supporting cross-reactivity.
• For Western blot, detect a band at the predicted molecular weight of 48 kDa; optimize loading and validate in relevant cell or tissue lysates.
• For ELISA, ensure coating antigen compatibility with the peptide immunogen and titrate antibody and detection reagents for optimal signal.
• As SMPD2 is a membrane-associated protein, use suitable extraction buffers and include protease inhibitors to maintain protein integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research labs with a seamless supply chain for IVD raw materials, from concept to clinic. This anti-SMPD2 polyclonal antibody supports studies in sphingolipid metabolism and lipid signaling. Beyond this product, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—all delivered with B2B reliability.