Anti-SMPD1 (KO Validated) Rabbit Monoclonal Antibody for WB, IF-F, IHC-P, ELISA - P17405

Core Product Specifications and Parameters

Biological Background: SMPD1 Function and Localization

• SMPD1 encodes sphingomyelin phosphodiesterase, also known as acid sphingomyelinase (aSMase). Alternative splicing produces isoforms; the protein exists as two enzymatic forms from alternative trafficking: one endolysosomal, the other secreted. Related references: PMID:20807762 PMID:21098024 PMID:9660788
• In the lysosomes, aSMase converts sphingomyelin to ceramide, playing a critical role in cholesterol export from intraendolysosomal membranes and exhibiting phospholipase C activities. Related references: PMID:20807762 PMID:21098024 PMID:25339683
• Modulates stress-induced apoptosis through the production of ceramide. Related references: PMID:8706124
• Secreted aSMase reorganizes plasma membrane by converting sphingomyelin to ceramide, and its secretion is upregulated by inflammatory mediators and pathogens, facilitating host defense and pathogen internalization. Related references: PMID:12563314 PMID:17303575 PMID:20807762 PMID:9393854
• During bacterial infection, the lysosomal form is released extracellularly and cleaved by CASP7, promoting plasma membrane repair and counteracting GSDMD perforin pores. Related references: PMID:21157428
• Subcellular localization includes lysosomes, lipid droplets, and secretion into the extracellular space.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation; the enzyme is zinc-dependent (metal-binding).

Experimental Guidance and Technical Tips

• The antibody is raised against a recombinant fragment encompassing amino acids 70–340 of human SMPD1, which may recognize epitopes within the catalytic domain. Consider validating specificity using knockout (KO) cell lysates where available.
• For Western blotting (WB), use reducing conditions and expect a band at approximately 70 kDa. Lysosomal enrichment or treatment with stressors may enhance detection.
• In immunofluorescence (IF-F) and immunohistochemistry (IHC-P), permeabilization and antigen retrieval are recommended. Co-staining with lysosomal markers can confirm subcellular localization.
• For ELISA, the antibody may be used as part of a matched pair; titrate and optimize conditions for your specific assay format.
• Cross-reactivity is reported for human and rat; for other species, validation is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop provider of IVD raw materials, supporting diagnostic developers and researchers with reliable antibodies, antigens, and custom sourcing. This anti-SMPD1 rabbit monoclonal antibody serves as a key tool for studying sphingolipid metabolism, lysosomal storage disorders, and pathogen-host interactions. Beyond this product, CamelBio offers validated antibody pairs, bulk reagents, and sourcing for rare targets to streamline your assay development from concept to clinic.