Anti-SMPD1 / ASM Polyclonal Antibody for WB, IF/ICC, ELISA - P17405

Core Product Specifications and Parameters

Biological Background: SMPD1 / ASM Function and Localization

• Encoded by the SMPD1 gene, acid sphingomyelinase (aSMase) is a sphingomyelin phosphodiesterase that catalyzes the conversion of sphingomyelin to ceramide. Related references: PMID:12563314, PMID:1840600, PMID:18815062

• The enzyme exists as two forms derived from a single precursor via alternative trafficking: one targeted to the endolysosomal compartment and the other released extracellularly; under stress conditions, lysosomal exocytosis becomes a major source of the secreted form. Related references: PMID:20807762, PMID:21098024, PMID:9660788, PMID:12563314, PMID:20530211

• Within lysosomes, aSMase converts sphingomyelin to ceramide, playing a critical role in cholesterol export from intraendolysosomal membranes and exhibiting phospholipase C activity towards 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol; it also modulates stress-induced apoptosis via ceramide production. Related references: PMID:20807762, PMID:21098024, PMID:25339683, PMID:8706124

• The secreted form reorganizes plasma membrane architecture by generating ceramide, thereby influencing cell signaling and host defense; its secretion is upregulated by stress and inflammatory mediators such as IL1B, IFNG, and TNF. Related references: PMID:12563314, PMID:17303575, PMID:20807762, PMID:9393854

• In wounded cells, the lysosomal form is released extracellularly in a Ca(2+)-dependent manner, promoting endocytosis and plasma membrane repair; a CASP7-cleaved form shows enhanced activity, further supporting membrane repair. Related references: PMID:20530211

• The enzyme is subverted by various pathogens: bacterial (e.g., P. aeruginosa, N. gonorrhoeae) and viral (e.g., SARS-CoV, SARS-CoV-2, Zaire ebolavirus) infections trigger its secretion, facilitating pathogen entry via ceramide-enriched membrane platforms.

• Mutations in SMPD1 lead to Niemann-Pick disease types A and B, characterized by sphingomyelin accumulation and neurodegeneration; the protein localizes to lysosomes, lipid droplets, and is secreted into the extracellular space.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 70-340, covering a large portion of the catalytic domain; the antibody is therefore expected to recognize both the precursor and mature forms of aSMase.
• For Western blotting, a band around 70 kDa is anticipated; however, glycosylation and processing may yield multiple forms, so validate in the relevant sample system.
• In immunofluorescence and immunocytochemistry applications, consider the dual localization of aSMase (lysosomes and secreted); appropriate fixation and permeabilization are recommended.
• ELISA applications may be optimized using recombinant aSMase as a standard; cross-reactivity with mouse and rat orthologs allows multispecies studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material procurement, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Supporting research into sphingolipid metabolism, apoptosis, and lysosomal storage disorders, we provide reliable access to critical reagents like this anti-SMPD1/ASM antibody. Whether you need rare target sourcing or technical consultation from concept to clinic, CamelBio streamlines your diagnostic development with a single, efficient supply chain.