Anti-SMPD1 / ASM Polyclonal Antibody for WB, IF/ICC, ELISA - P17405

Core Product Specifications and Parameters

Biological Background: SMPD1 / ASM Function and Localization

• SMPD1/ASM is a lysosomal hydrolase that converts sphingomyelin into ceramide, a lipid mediator involved in apoptosis, differentiation, and cell signaling. Related references: PMID:12563314, PMID:1840600, PMID:18815062
• It exists as two enzymatic forms: one targeted to the endolysosomal compartment and the other released extracellularly, with lysosomal exocytosis being a major source of the secretory form. Related references: PMID:20807762, PMID:21098024, PMID:9660788, PMID:12563314, PMID:20530211
• In lysosomes, it converts sphingomyelin to ceramide and plays a role in cholesterol export from intraendolysosomal membranes; it also has phospholipase C activity. Related references: PMID:20807762, PMID:21098024, PMID:25339683
• Secreted ASM reorganizes plasma membrane rafts by generating ceramide, which is crucial for host defense against pathogens like Pseudomonas aeruginosa. Related references: PMID:12563314, PMID:17303575, PMID:20807762, PMID:9393854
• Cleavage by CASP7 in the extracellular space yields a more active form that enhances ceramide production and plasma membrane repair during bacterial infection. Related references: PMID:21157428
• ASM activation is hijacked by viruses such as SARS-CoV and SARS-CoV-2 to facilitate infection, and by bacteria like N. gonorrhoeae.
• Genetic defects in SMPD1 lead to Niemann-Pick disease, a lysosomal storage disorder characterized by sphingomyelin accumulation.

Experimental Guidance and Technical Tips

• The immunogen covers a large central region (aa 70-340) of human SMPD1, suggesting the antibody may recognize both precursor and mature forms. Consider using protease inhibitors during sample preparation to maintain full-length protein integrity.
• For Western blot, a band around 70 kDa is expected. Verify specificity by using appropriate positive controls (e.g., human, mouse, or rat cell lysates known to express SMPD1) and include negative controls.
• In immunofluorescence/ICC, staining may be observed in lysosomal compartments; co-staining with lysosomal markers is recommended to confirm localization.
• The antibody cross-reacts with mouse and rat; therefore, it can be used in rodent model studies, but always validate in the relevant species and tissue context.
• ELISA protocols should be optimized with recombinant SMPD1 protein or appropriate coating antigens; consider pairing with matched detection antibodies for sandwich ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, streamlining access to critical reagents like this anti-SMPD1/ASM antibody for research into sphingolipid metabolism, Niemann-Pick disease, and host-pathogen interactions. From validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents and custom rare-target sourcing, we support your workflow from concept to clinic. Connect with our team to learn how we can fulfill your specific antibody and ancillary reagent requirements.