Anti-SMOC1 Polyclonal Antibody for WB, IHC-P, ELISA - Q9H4F8

Core Product Specifications and Parameters

Biological Background: SMOC1 Function and Localization

• SMOC1 (SPARC-related modular calcium-binding protein 1), also known as secreted modular calcium-binding protein 1, is a member of the SPARC family of matricellular proteins.
• It plays essential roles in eye and limb development and is a probable regulator of osteoblast differentiation.
• The protein is secreted into the extracellular space, localizing to the extracellular matrix and basement membrane.
• Widely expressed across tissues, with the strongest signal in ovary; absent in spleen.
• Post-translational modifications include glycosylation and disulfide bond formation.
• Key functional keywords: calcium-binding, metal-binding, developmental protein; disease-associated variants linked to microphthalmia.
• Alternative splicing generates multiple isoforms of SMOC1.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 27-250 of human SMOC1; for WB, consider titrating the antibody in relevant cell lysates (e.g., ovarian tissue or osteoblast lineages).
• For IHC-P, validate staining in tissue sections with known high expression, such as ovary; note that SMOC1 is secreted, so optimization of fixation may be required.
• ELISA can be employed to detect recombinant or native SMOC1; include appropriate controls and confirm cross-reactivity with mouse samples if needed.
• As a polyclonal antibody, lot-to-lot variability should be assessed, and dilutions should be optimized for each application.

CamelBio: Your One-Stop Sourcing Bridge

This SMOC1 antibody serves as a reliable tool for research in developmental biology, extracellular matrix biology, and bone differentiation studies. CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials—including validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing—supporting seamless project transition from concept to clinic.