Anti-SMN2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q16637

Core Product Specifications and Parameters

Biological Background: SMN2 Function and Localization

• SMN2 encodes the survival motor neuron protein (Gemin-1), a key scaffold in the SMN complex that catalyzes snRNP assembly, essential for pre-mRNA splicing. Related references: PMID:18984161 PMID:9845364
• It acts as a structural backbone, cooperating with GEMIN2 to accept pre-assembled Sm proteins from the CLNS1A chaperone and promote their loading onto snRNA, triggering eviction of the SMN complex to complete core snRNP assembly. Related references: PMID:17178713 PMID:21816274 PMID:22101937 PMID:31799625
• SMN2 ensures correct splicing of U12 intron-containing genes, critical for motor and proprioceptive neuron development. Related references: PMID:23063131
• The protein resolves R-loops at transcription termination sites, facilitating proper termination by RNA polymerase II. Related references: PMID:26700805
• Subcellularly, SMN2 localizes to nuclear gems, Cajal bodies, cytoplasmic granules, neuronal processes, axon projections, and sarcomeric Z lines.
• It is subject to acetylation, phosphorylation, and ubiquitination and undergoes alternative splicing.
• Widely expressed, with high levels in brain, kidney, liver, and spinal cord; moderate levels in skeletal and cardiac muscle; lower levels in fibroblasts and lymphocytes.

Experimental Guidance and Technical Tips

• In Western blot, the detected band is expected at approximately 32 kDa. Confirm specificity using positive and negative control lysates.
• For immunofluorescence/ICC, optimization of fixation and permeabilization may be required, as SMN2 localizes to both nuclear foci (gems/Cajal bodies) and cytoplasmic compartments.
• This antibody cross-reacts with mouse and rat; users should validate performance in their specific experimental systems.
• Given the high sequence similarity between SMN1 and SMN2, consider strategies to differentiate if isoform specificity is critical.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop partner for IVD raw materials, CamelBio supplies high-quality antibodies like this anti-SMN2 polyclonal antibody, alongside optimized reagent sets for splicing and neuromuscular disease research. Our procurement services cover everything from monoclonal/polyclonal antibodies to ancillary reagents, ensuring seamless sourcing for your diagnostic and research programs. Contact us to explore tailored supply solutions for your project needs.