Anti-SMN2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q16637

Core Product Specifications and Parameters

Biological Background: Survival motor neuron protein Function and Localization

• Survival motor neuron protein (SMN; also known as Gemin-1) is encoded by the SMN1 and SMN2 genes and acts as a structural backbone of the SMN complex, orchestrating snRNP biogenesis.
• The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), essential building blocks of the spliceosome, thereby playing a critical role in pre-mRNA splicing. Related references:
  PMID:18984161 PMID:9845364
• In the cytosol, the SMN complex accepts Sm proteins from the CLNS1A chaperone; SMN1, together with GEMIN2, gathers the Sm subunits onto snRNA, and subsequent snRNA binding triggers SMN complex eviction to complete core snRNP assembly. Related references:
  PMID:17178713 PMID:21816274 PMID:22101937 PMID:31799625
• Ensures correct splicing of U12 intron-containing genes, which is important for normal motor and proprioceptive neuron development. Related references:
  PMID:23063131
• Required for resolving RNA-DNA hybrids (R-loops) created by RNA polymerase II in transcription terminal regions, facilitating proper transcription termination. Related references:
  PMID:26700805
• Localizes to nuclear gems, Cajal bodies, cytoplasm, and neuronal projections; also found at myofibril Z-lines. Widely expressed with high levels in brain, kidney, liver, and spinal cord; present in osteoclasts and mononuclear cells. Keywords indicate involvement in neurodegeneration and neurogenesis, and the protein undergoes acetylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1–197) common to SMN1 and SMN2; the polyclonal antibody likely recognizes both isoforms—validate isoform specificity in your model if required.
• For Western blotting, a major band at approximately 32 kDa is expected; confirm the molecular weight and antibody specificity in the relevant sample system.
• In immunofluorescence, anticipate nuclear gem/Cajal body and cytoplasmic staining patterns; use appropriate cellular markers for co-localization.
• For immunoprecipitation, test the antibody under native conditions; co-immunoprecipitation of associated Gemins may be observed.
• For ELISA, the antibody is validated; optimize conditions with recombinant SMN or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, supporting diagnostic manufacturers with validated antibodies, bulk reagents, and rare-target procurement. This SMN2 polyclonal antibody exemplifies our capability to deliver quality reagents for neuroscience and genetic disease research. Contact us to explore tailored solutions for your splicing or motor neuron disease projects.