Anti-SMN1 Polyclonal Antibody for WB, IHC-P, ELISA - Q16637

Core Product Specifications and Parameters

Biological Background: SMN1 Function and Localization

• The SMN complex, with SMN1 as its structural backbone, catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the fundamental units of the spliceosome, thereby playing a critical role in pre-mRNA splicing. Related references: PMID:18984161, PMID:9845364
• Within the SMN complex, SMN1 acts together with GEMIN2 to gather Sm core proteins (SNRPB, SNRPD1-3, SNRPE-G) from the chaperone CLNS1A, forming an intermediate that primes snRNP assembly. Related references: PMID:17178713, PMID:21816274, PMID:22101937
• Binding of snRNA to the nascent Sm core triggers eviction of the SMN complex, allowing final incorporation of SNRPD3 and SNRPB to complete the core snRNP. Related references: PMID:31799625
• SMN1 is essential for correct splicing of U12 intron-containing genes, a process important for normal motor and proprioceptive neuron development. Related references: PMID:23063131
• The protein also resolves R-loops formed during RNA polymerase II transcription termination, ensuring proper transcriptional control. Related references: PMID:26700805
• SMN1 localizes to nuclear structures including gems and Cajal bodies, as well as the cytoplasm, where it is found in granules and associates with the sarcomeric Z line and neuronal projections.
• Expressed across many tissues, with highest levels in brain, kidney, liver, spinal cord, and skeletal muscle, and lower levels in fibroblasts and lymphocytes.
• The protein undergoes various post-translational modifications, including phosphorylation, acetylation, and ubiquitin-like conjugation, which may regulate its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 11–169) covers the N-terminal region shared by SMN1 and its highly homologous counterpart SMN2; the antibody may recognize both isoforms. Consider validating specificity if isoform discrimination is required.
• For Western blot, the expected molecular weight is approximately 32 kDa. Use appropriate positive controls (e.g., HeLa, HEK293, or neuronal cell lines) and validate detection in the relevant sample system.
• In immunohistochemistry (IHC-P), SMN1 localizes to nuclear gems and Cajal bodies, offering a distinctive punctate staining pattern. Antigen retrieval and antibody dilution should be optimized for the tissue of interest.
• For ELISA applications, coat with recombinant SMN1 protein or cell lysates, and include proper negative controls to confirm signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, connecting diagnostic manufacturers and research laboratories with high-quality reagents like this anti-SMN1 antibody. Specializing in the neurodegenerative disease and RNA processing fields, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—streamlining your path from concept to clinic.