Anti-SMMHC/MYH11 Rabbit Polyclonal Antibody for WB - P35749

Core Product Specifications and Parameters

Biological Background: SMMHC/MYH11 Function and Localization

• SMMHC/MYH11, also known as myosin-11 or smooth muscle myosin heavy chain, is a major contractile protein encoded by the MYH11 gene.
• It is a member of the myosin heavy chain family, functioning as a motor protein that converts chemical energy (ATP) into mechanical force during smooth muscle contraction.
• Alternate splicing generates multiple isoforms; isoform 1 is predominantly expressed in slowly contracting tonic smooth muscles.
• The protein is localized to melanosomes and is a component of the thick filaments in smooth muscle cells.
• It contains conserved domains for actin-binding, ATP-binding, and calmodulin-binding, enabling its role in muscle contraction.
• Post-translational modifications include methylation and phosphorylation, which may regulate its activity and interactions.
• Tissue expression is detected in vascular smooth muscle (umbilical artery) and visceral smooth muscle (bladder, esophagus, trachea).
• Genetic variants and chromosomal rearrangements involving MYH11 are associated with aortic aneurysm and have been implicated as a proto-oncogene in certain leukemias.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 1680-1979) of human SMMHC, which is conserved across human, mouse, and rat—consistent with the reported cross-reactivity.
• For Western blotting, expect a band around 227 kDa; validate in a relevant smooth muscle tissue or cell lysate.
• This polyclonal antibody may be suitable for immunofluorescence on paraffin sections (IF-P) and ELISA; confirm optimal dilutions and conditions in your experimental system.
• Consider using non-committal statements to avoid overpromising: "may detect," "suggested for," "validate in your assay."

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-SMMHC/MYH11 antibody supports research in smooth muscle physiology and associated disorders. Explore our validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for your diagnostic development needs.