Anti-SMMHC/MYH11 Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P35749

Core Product Specifications and Parameters

Biological Background: MYH11 (Myosin-11) Function and Localization

• Encoded by the MYH11 gene, myosin-11 (also known as smooth muscle myosin heavy chain, SMMHC) is a 227 kDa motor protein that assembles into thick filaments and generates mechanical force through actin-based ATP hydrolysis.
• Functions as the primary contractile protein in smooth muscle, mediating muscle contraction through cyclic interactions with actin filaments.
• Isoform 1 is predominantly expressed in slowly contracting tonic smooth muscles, such as those in the umbilical artery, bladder, esophagus, and trachea.
• Localizes to melanosomes, in addition to its expected thick filament localization, suggesting a role in organelle transport or melanosome function.
• Post-translational modifications include phosphorylation and methylation, which may regulate motor activity and filament assembly.
• Classified under the myosin family and contains conserved actin-binding, ATP-binding, and calmodulin-binding domains characteristic of myosin heavy chains.
• Associated with human pathological conditions: chromosomal rearrangements involving MYH11 have been implicated as proto-oncogenic events, and mutations are linked to aortic aneurysm.

Experimental Guidance and Technical Tips

• For Western blotting, consider using a low-percentage SDS-PAGE gel (e.g., 6–8%) and extended wet transfer times to accommodate the large 227 kDa protein.
• Validate detection in relevant smooth muscle-containing tissues (e.g., bladder, uterus, trachea) for IHC-P and IF-P; ensure appropriate positive and negative controls.
• The immunogen corresponds to residues 1160–1313 of human SMMHC/MYH11; verify cross-reactivity in mouse and rat samples due to sequence divergence in this region.
• For ELISA, use the recombinant immunogen or purified protein to establish antibody titer and sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-MYH11 antibody supports smooth muscle biology and cardiovascular disease research, complementing our wide catalog for diagnostic developers. For rare target sourcing or custom antibody development, our team provides tailored support from concept to clinic.