Anti-SMG7 Rabbit Polyclonal Antibody for WB, ELISA - Q92540

Core Product Specifications and Parameters

Biological Background: SMG7 Function and Localization

• SMG7 (hSMG-7, EST1C, SGA56M, C1orf16) is a nonsense-mediated mRNA decay (NMD) factor that plays a central role in mRNA surveillance and quality control.
• It recruits UPF1 to cytoplasmic mRNA decay bodies, which are sites of mRNA degradation.
• SMG7 acts together with SMG5 to link the NMD machinery to exonucleolytic mRNA decay pathways.
• It serves as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation, a critical step in NMD.
• Subcellular localization includes both the cytoplasm and nucleus.
• The protein features multiple TPR repeats and undergoes post-translational modifications such as phosphorylation and acetylation.
• Alternative splicing generates multiple isoforms of SMG7.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (amino acids 1–300), which may be suitable for detecting full-length SMG7 across species.
• For Western blot, consider the predicted molecular weight of approximately 127 kDa and validate in the relevant sample system.
• ELISA applications may be optimized using the recombinant protein as a standard; cross-reactivity with mouse and rat SMG7 should be confirmed under experimental conditions.
• Due to the presence of isoforms and post-translational modifications, band patterns or signal intensities may vary; include appropriate controls and possibly phosphatase inhibitors.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare target materials. This SMG7 antibody supports research into nonsense-mediated mRNA decay and post-transcriptional gene regulation, and we can supply custom quantities and related reagents to streamline your assay development.