Anti-SMEK1 Polyclonal Antibody for WB, IHC-P, ELISA - Q6IN85

Core Product Specifications and Parameters

Biological Background: SMEK1 Function and Localization

• Serine/threonine-protein phosphatase 4 regulatory subunit 3A (PPP4R3A), also known as SMEK homolog 1, is encoded by the PPP4R3A gene in humans. Alternative names include PP4R3A, FLFL1, and KIAA2010.
• Acts as a regulatory subunit of protein phosphatase 4 (PP4) and may modulate PPP4C catalytic activity at centrosomal microtubule organizing centers.
• In the PP4 complex (PPP4C-PPP4R2-PPP4R3A), it specifically dephosphorylates γ-H2AX (phosphorylated at Ser-140), a process essential for DNA double-strand break repair during DNA replication.
• Localizes to the cytoplasm, cytoskeleton (centrosome), and nucleus, consistent with its role in DNA damage signaling and centrosomal function.
• UniProt keywords highlight its nature as a phosphoprotein, acetylated protein, and the presence of alternative splicing events.
• A 3D-structure is available, providing insights into its molecular interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 395–594 of human SMEK1 (isoform 2); validating antibody specificity in the relevant sample system is recommended.
• For Western blotting, the predicted molecular weight is approximately 95 kDa; additional bands may arise from isoforms or post-translational modifications.
• In immunohistochemistry (paraffin), antigen retrieval and antibody titration should be optimized for each tissue.
• Cross-reactivity with mouse and rat has been reported by the supplier; additional verification in the target species/tissue is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, technical services, and consulting—from concept to clinic. For your SMEK1 research needs in DNA damage repair and phosphatase signaling, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our sourcing capabilities extend to rare-target raw materials, streamlining your procurement process.