Anti-SMCHD1 Polyclonal Antibody for WB, IF/ICC, ELISA - A6NHR9

Core Product Specifications and Parameters

Biological Background: SMCHD1 Function and Localization

• SMCHD1 (Structural maintenance of chromosomes flexible hinge domain-containing protein 1), also known as BAMS or FSHD2, is a non-canonical member of the structural maintenance of chromosomes (SMC) protein family.
• As a chromatin regulator, SMCHD1 promotes heterochromatin formation on both autosomes and the X chromosome, probably by mediating the merge of chromatin compartments.
• It plays a key role in chromosome X inactivation in females by promoting the spreading of heterochromatin; it is recruited by Xist RNA and facilitates Xist RNA spreading across the inactivated X chromosome. Related references: PMID:23542155
• SMCHD1 is required for silencing of a subset of clustered autosomal loci in somatic cells, such as the DUX4 locus—a function linked to facioscapulohumeral muscular dystrophy type 2 (FSHD2). Related references: PMID:23143600
• The protein possesses ATPase activity and may participate in ATP‑dependent structural manipulation of chromatin to regulate gene expression. Related references: PMID:29748383
• SMCHD1 contributes to DNA repair: it localizes to sites of DNA double‑strand breaks and promotes repair via non‑homologous end joining (NHEJ) while inhibiting homologous recombination (HR). Related references: PMID:24790221 PMID:25294876
• Subcellularly, SMCHD1 is localized to chromosomes, consistent with its functions in chromatin organization and DNA repair.
• Post‑translational modifications indicated by UniProt keywords include acetylation, phosphorylation, and ubiquitin conjugation; alternative splicing may generate multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 1756‑2005 in the C‑terminal region; consider potential isoform‑specific detection and verify reactivity with your target sequence.
• For Western blot, expect a band at approximately 226 kDa. Use high‑molecular‑weight markers and optimize transfer conditions for large proteins.
• In immunofluorescence/immunocytochemistry (IF/ICC), SMCHD1 should localize to chromatin‑rich regions; include appropriate controls for nuclear staining.
• ELISA applications may be developed using recombinant SMCHD1 or cell lysates; validate linearity and specificity with your sample set.
• As a rabbit polyclonal antibody, monitor inter‑lot consistency if used in quantitative or regulated assays.
• Cross‑reactivity with rat is predicted based on sequence similarity; confirm performance in your model system.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one‑stop channel for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target sourcing. For SMCHD1‑focused projects in epigenetic silencing, chromatin biology, and DNA repair, we deliver the critical raw materials and technical support needed to advance your assays from concept to clinic.