Anti-SMC4 Monoclonal Antibody for WB, IF/ICC, ELISA - Q9NTJ3

Core Product Specifications and Parameters

Biological Background: SMC4 Function and Localization

• SMC4 (Structural Maintenance of Chromosomes protein 4) is a central component of the condensin complex, also known as chromosome-associated polypeptide C (hCAP-C) or XCAP-C homolog, with aliases CAPC, SMC-4, SMC4L1.
• It plays a critical role in converting interphase chromatin into mitotic-like condensed chromosomes, introducing positive supercoils into relaxed DNA in the presence of type I topoisomerases and converting nicked DNA into positive knotted forms with type II topoisomerases.
• SMC4 belongs to the SMC (Structural Maintenance of Chromosomes) family, which includes core proteins involved in chromosome organization, cohesion, and condensation.
• The protein localizes to the nucleus, cytoplasm, and chromosomes, consistent with its dynamic role during the cell cycle.
• Post-translational modifications include acetylation and phosphorylation, as indicated by UniProt keywords, suggesting regulatory mechanisms in cell division.
• Widely expressed with higher levels in testis, colon, and thymus, reflecting its essential role in proliferating tissues.
• Keywords also highlight ATP-binding and nucleotide-binding domains, supporting its energy-dependent condensin functions.

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was generated against a synthetic peptide corresponding to amino acids 1189-1288 of human SMC4 (Q9NTJ3). Due to sequence conservation, cross-reactivity with mouse and rat SMC4 is expected, but we recommend verifying in relevant tissue or cell lysates.
• Western blot (WB): The predicted molecular weight is ~147 kDa. Use a suitable gel percentage (e.g., 6-8% SDS-PAGE) and appropriate transfer conditions for high-molecular-weight proteins.
• Immunofluorescence/Immunocytochemistry (IF/ICC): SMC4 localizes to the nucleus and chromosomes; standard fixation and permeabilization protocols should be effective. Validate antibody specificity using positive controls such as proliferating cells (e.g., HeLa) where condensin activity is high.
• ELISA: Suitable for detecting SMC4 in sandwich or indirect ELISA formats; optimization of coating antigen and antibody concentration is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-SMC4 antibody supports research into chromosome condensation and cell cycle regulation. Our portfolio includes validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your IVD development.