Anti-SMC3 Rabbit Polyclonal Antibody (SMC3 pAb) for WB, IP, ELISA - Q9UQE7

Core Product Specifications and Parameters

Biological Background: SMC3 Function and Localization

• SMC3 (Structural maintenance of chromosomes protein 3) is a central component of the cohesin complex, which is essential for sister chromatid cohesion during the cell cycle. The cohesin complex may form a ring-like structure that entraps sister chromatids, and is cleaved at anaphase to allow chromosome segregation.
• The cohesin complex plays critical roles in DNA replication and DNA repair processes. SMC3 is involved in the repair of DNA double-strand breaks by homologous recombination.
• SMC3 is also important for spindle pole assembly during mitosis and contributes to chromosome movement, ensuring proper mitotic progression.
• Alternative names for SMC3 include Bamacan (Basement membrane-associated chondroitin proteoglycan), Chondroitin sulfate proteoglycan 6, and Chromosome-associated polypeptide (hCAP). It belongs to the SMC (structural maintenance of chromosomes) protein family.
• The protein localizes to the nucleus, associating with chromosomes and concentrating at the centromere region during mitosis.
• Post-translational modifications of SMC3 include acetylation and phosphorylation, which regulate cohesin complex dynamics and function. The protein contains an ATP-binding domain, and ATP hydrolysis is required for its activities.
• SMC3 is ubiquitously expressed and is subject to ubiquitin-like modifications. Mutations in SMC3 are associated with Cornelia de Lange syndrome (CDLS3), a developmental disorder characterized by intellectual disability and growth retardation.
• The protein has a molecular weight of approximately 142 kDa and forms heterodimers with SMC1 as part of the cohesin complex.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to the C-terminal region (amino acids 909-1199) of human SMC3. Researchers should confirm reactivity by using appropriate positive and negative controls, such as lysates from cell lines expressing SMC3 and knockout cells.
• For Western blot (WB), a band near 142 kDa is expected. Due to the large size of SMC3, use a gradient gel (e.g., 4-20%) and optimize transfer conditions for high-molecular-weight proteins.
• This antibody has been reported for immunoprecipitation (IP). When performing IP, consider using a cross-linking step if co-immunoprecipitation of interacting partners is desired, as harsh elution conditions may be required.
• For ELISA applications, validate the antibody pair and antigen recognition in the specific buffer and plate format intended for your assay.
• Although the antibody shows cross-reactivity with mouse SMC3, verify performance in your experimental model, as differences in tissue or cell type may affect results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research communities with comprehensive one-stop access to IVD raw materials, including validated antibodies for chromosome biology, cell cycle, and DNA repair targets like SMC3. We offer polyclonal and monoclonal antibody supply, optimized antibody pairs, and bulk ancillary reagents to streamline your assay development. For rare or specialized targets, CamelBio provides tailored raw-material sourcing and technical consulting to help bring your projects from concept to clinic.