Anti-SMC3 Rabbit Monoclonal Antibody for WB, IF/ICC, IP, ELISA - Q9UQE7

Core Product Specifications and Parameters

Biological Background: SMC3 Function and Localization

• SMC3 (Structural maintenance of chromosomes protein 3), also known as Bamacan, BMH, HCAP, CSPG6, and SMC3L1, is a central component of the cohesin complex.
• The cohesin complex, which includes SMC3, is required for sister chromatid cohesion, forming a ring-like structure that traps chromatids until anaphase.
• Cohesion established by SMC3 is essential for proper chromosome segregation, DNA replication, and DNA repair.
• SMC3 participates in spindle pole assembly and chromosome movement during mitosis.
• It is localized to the nucleus, specifically at chromosomes and centromeres.
• Post-translational modifications of SMC3 include acetylation and phosphorylation, and mutations are associated with Cornelia de Lange syndrome 3 (CDLS3), reflecting keywords such as disease variant and intellectual disability.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning amino acids 1118-1217 near the C-terminus of human SMC3; this immunogen design may contribute to target specificity.
• For WB, anticipate a band at approximately 142 kDa; consider using a low-percentage gel and appropriate protein standards.
• In IF/ICC experiments, ensure sufficient permeabilization to allow nuclear antigen detection.
• For IP, the large size of SMC3 (142 kDa) may require optimization of lysis and binding conditions.
• Cross-reactivity with mouse and rat has been indicated; validate performance in your sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering this anti-SMC3 antibody ideal for investigating cohesin function, chromosome dynamics, and related disease mechanisms. Alongside validated antibodies, we supply bulk ancillary reagents and can source rare target raw materials to support your diagnostic or research workflows. From initial development to clinical application, CamelBio's technical services ensure seamless project progression.