Anti-SMC1 Rabbit Polyclonal Antibody for WB and ELISA - Q14683

Core Product Specifications and Parameters

Biological Background: SMC1 Function and Localization

• Structural maintenance of chromosomes protein 1A (SMC1A) is a core component of the cohesin complex, which ensures proper sister chromatid cohesion after DNA replication.
• The cohesin complex forms a ring-like structure that topologically entraps sister chromatids; cleavage of the complex at anaphase triggers chromatid separation and segregation.
• SMC1A participates in DNA damage repair pathways, interacting with BRCA1 and being phosphorylated by ATM/ATR, thus acting as a downstream effector in both ATM/NBS1 and ATR/MSH2 branches of the S-phase checkpoint.
• Additionally, SMC1A contributes to spindle pole assembly during mitosis, underscoring its multifaceted role in chromosome dynamics.
• Localized mainly in the nucleus, SMC1A associates with chromatin at centromeres and kinetochores during cell division.
• Post-translational modifications such as acetylation, phosphorylation, and ubiquitination regulate SMC1A activity, as highlighted by its ATP-binding and phosphoprotein motifs.
• Mutations in SMC1A are associated with human diseases including Cornelia de Lange syndrome, developmental and epileptic encephalopathy, and other neurodevelopmental disorders, consistent with its Disease variant keyword.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-130) of human SMC1; consider that antigenic determinants may be conserved across species, supporting the observed cross-reactivity with mouse and rat.
• For Western blotting, use a low-percentage polyacrylamide gel (e.g., 8%) to effectively resolve the 143 kDa protein; optimize transfer conditions for high-molecular-weight proteins.
• Validate the antibody's performance in your specific sample type and application by including appropriate positive and negative controls; endogenous expression levels can vary by cell line or tissue.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop provider of IVD raw materials, technical services, and consulting, supporting diagnostic manufacturers and researchers from concept to clinic. This SMC1 antibody is part of our extensive portfolio for chromosome biology, cell cycle, and DNA repair research. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to accelerate your assay development.