Anti-SMC1 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q14683

Core Product Specifications and Parameters

Biological Background: SMC1 Function and Localization

• SMC1A encodes structural maintenance of chromosomes protein 1A, a central component of the cohesin complex essential for sister chromatid cohesion after DNA replication.
• The cohesin complex is thought to form a ring-like structure that traps sister chromatids, and its cleavage at anaphase triggers chromatid segregation, enabling proper chromosome distribution.
• Beyond cohesion, SMC1A participates in DNA repair by interacting with BRCA1 and undergoing phosphorylation by ATM, acting in the ATM/NBS1 branch of the S-phase checkpoint.
• SMC1A also plays a role in spindle pole assembly during mitosis, linking chromosome cohesion with mitotic spindle organization.
• Subcellularly, SMC1A localizes to the nucleus and is enriched at chromosomes, particularly at centromeres and kinetochores, aligning with its mitotic functions.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (by SUMO), which modulate its activity and interactions.
• Mutations in SMC1A are associated with Cornelia de Lange syndrome (CDLS2), developmental and epileptic encephalopathy (DEE85, EIEE85), highlighting its critical developmental roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 1134-1233); the antibody may detect full-length SMC1 (~143 kDa) and potentially cleaved fragments in Western blot.
• For IF/ICC, expect nuclear and chromosomal staining, with enrichment at centromeres and kinetochores; validate fixation and permeabilization conditions.
• Cross-reactivity with mouse is reported; validate reactivity in your specific model system before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw-material sourcing, supporting researchers and manufacturers from concept to clinic. This anti-SMC1 antibody enables robust detection in chromosome biology, cell cycle, and DNA repair studies. Beyond this product, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to streamline your assay development and scale-up.