Anti-SMARCD3 Rabbit Polyclonal Antibody for WB, ELISA - Q6STE5

Core Product Specifications and Parameters

Biological Background: SMARCD3 Function and Localization

• SMARCD3 (BAF60C) is a subunit of SWI/SNF chromatin remodeling complexes, involved in ATP-dependent alteration of DNA-histone contacts to regulate DNA accessibility.
• It stimulates nuclear receptor-mediated transcription, playing a dual role in gene activation and repression by remodeling nucleosome structure.
• In neural development, SMARCD3 is a component of neural progenitor-specific (npBAF) and neuron-specific (nBAF) complexes; the subunit switch from npBAF to nBAF is critical for the transition from proliferative progenitors to postmitotic neurons.
• The protein localizes to the nucleus, consistent with its function in chromatin organization and transcriptional regulation.
• Alternative splicing generates multiple isoforms; isoform 1 and isoform 2 exhibit broad tissue distribution, including brain, heart, kidney, placenta, prostate, spleen, testis, thyroid, trachea, uterus, with isoform 1 additionally expressed in skeletal muscle and adipose tissue.
• Post-translational modifications include acetylation and phosphorylation, as identified in proteomic studies, which may modulate its activity or interactions.
• Classified under keywords: acetylation, alternative splicing, chromatin regulator, neurogenesis, nucleus, phosphoprotein, and transcription regulation.

Experimental Guidance and Technical Tips

• For Western blotting, anticipate a specific band at approximately 55 kDa in human, mouse, or rat cell/tissue lysates; validate under reducing conditions.
• Since the immunogen covers amino acids 275–440, epitope recognition may be affected by isoform-specific sequences or post-translational modifications; consider testing the antibody in relevant cell models expressing the target.
• As a polyclonal antibody, lot-to-lot consistency may vary; titrate primary antibody concentration and include positive controls (e.g., SMARCD3-overexpressing lysates) and negative controls (e.g., siRNA knockdown samples).
• For ELISA, optimize coating antigen (recombinant SMARCD3 or peptide) concentration and use appropriate blocking buffers to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier one-stop platform for IVD raw material sourcing, supplying validated antibody pairs, bulk ancillary reagents, and custom procurement services. This anti-SMARCD3 polyclonal antibody supports research into chromatin remodeling, transcription regulation, and neurogenesis. Our integrated supply chain and technical support ensure reliable access to high-quality materials for your diagnostic and research projects.