Anti-SMARCD2 Polyclonal Antibody for WB, IHC-P, ELISA - Q92925

Core Product Specifications and Parameters

Biological Background: SMARCD2 (BAF60B) Function and Localization

• SMARCD2 (also known as BAF60B) encodes the 60 kDa BRG-1/Brm-associated factor subunit B, a core component of the SWI/SNF chromatin remodeling complex.
• The protein plays a key role in altering DNA-nucleosome topology to facilitate transcriptional activation and repression of specific genes.
  Related references:
  PMID:22952240 PMID:26601204
• SMARCD2 is a critical regulator of myeloid differentiation, controlling granulocytopoiesis and the expression of genes involved in neutrophil granule formation.
  Related references:
  PMID:28369036
• As part of the SWI/SNF family, it shares homology with yeast Rsc6p and operates within multi-subunit complexes that remodel nucleosomes in an ATP-dependent fashion.
• The protein localizes exclusively to the nucleus, consistent with its role in chromatin regulation.
• Isoform 2 exhibits tissue-specific expression in the pancreas, suggesting specialized functions.
• Post-translational modifications include phosphorylation, methylation, and isopeptide bond formation, potentially modulating its activity and interactions.
• Functional keyword annotations from UniProt: Alternative splicing, Chromatin regulator, Isopeptide bond, Methylation, Nucleus, Phosphoprotein, Proteomics identification, Reference proteome, Transcription, Transcription regulation, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 454–531 of human SMARCD2. When performing western blot, the predicted molecular weight is approximately 59 kDa; always validate in the relevant sample system.
• For immunohistochemistry (IHC-P) and ELISA, optimize primary antibody dilution and antigen retrieval conditions empirically to ensure sensitivity and specificity.
• Given the antibody’s reported reactivity with human, mouse, and rat, consider cross-reactivity verification in your specific model before quantitative assays.
• As a polyclonal antibody, lot-to-lot consistency should be monitored, and involving appropriate controls is recommended for reproducibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-SMARCD2 polyclonal antibody joins our chromatin remodeling and transcription regulation product line, enabling robust assay development. Beyond standard catalog offerings, we specialize in sourcing rare-target raw materials and providing technical consulting — helping partners move from concept to clinic with confidence.