Anti-SMARCC2 Polyclonal Antibody for WB, ELISA - Q8TAQ2

Core Product Specifications and Parameters

Biological Background: SMARCC2 Function and Localization

• SMARCC2, also known as BAF170, is a core subunit of the SWI/SNF chromatin remodeling complex, belonging to the SMARCC family and found in both canonical BAF and PBAF complexes.
• It is involved in transcriptional activation and repression by altering DNA-nucleosome topology in an ATP-dependent manner (PubMed:11018012). It can stimulate the ATPase activity of the catalytic subunit of these complexes (PubMed:10078207). Related references: PMID:11018012 PMID:10078207
• SMARCC2 may be required for CoREST-dependent repression of neuronal gene promoters in non-neuronal cells (PubMed:12192000). Related references: PMID:12192000
• It is a component of both the neural progenitors-specific (npBAF) and neuron-specific (nBAF) chromatin remodeling complexes, which undergo subunit switching during neural differentiation to regulate stem cell self-renewal and dendrite growth, respectively (By similarity).
• SMARCC2 is a critical regulator of myeloid differentiation, controlling granulocytopoiesis and neutrophil granule formation gene expression (By similarity).
• Localizes exclusively to the nucleus, and is ubiquitously expressed. Post-translational modifications include acetylation, phosphorylation, ADP-ribosylation, and ubiquitination, while disease-associated variants are linked to intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 150–250 of human SMARCC2. Align this region with mouse and rat sequences to confirm cross-reactivity expectations.
• For Western blotting, SMARCC2 has a predicted molecular weight of ~133 kDa. Use a low-percentage acrylamide gel (e.g., 8%) and extended transfer times for efficient high-molecular-weight protein transfer.
• When performing ELISA, optimize antibody and antigen concentrations via checkerboard titration. Include appropriate positive (e.g., recombinant SMARCC2) and negative controls to verify specificity.
• Consider endogenous expression levels: SMARCC2 is ubiquitously expressed but may vary across cell types and conditions; validate detection in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering diagnostic manufacturers and researchers a single point of access to validated reagents and supply chain solutions. This anti-SMARCC2 polyclonal antibody supports chromatin remodeling, neurogenesis, and myeloid differentiation studies. Beyond individual antibodies, CamelBio can supply optimized antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and customized sourcing for rare-target raw materials—bridging the gap from concept to clinical application.