Anti-SMARCC1/BAF155 Monoclonal Antibody for WB, IF/ICC, ELISA, ChIP - Q92922

Core Product Specifications and Parameters

Biological Background: SMARCC1/BAF155 Function and Localization

• SMARCC1 (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1), also known as BAF155, BRG1-associated factor 155, is a core subunit of the SWI/SNF chromatin remodeling complexes.
• It plays a critical role in transcriptional activation and repression by altering DNA-histone contacts in an ATP-dependent manner and may stimulate the ATPase activity of the catalytic subunit. Related references: PMID:10078207 PMID:29374058
• SMARCC1 is a component of neural progenitor-specific (npBAF) and neuron-specific (nBAF) complexes, where subunit switching during differentiation is essential for neural stem cell self-renewal and dendrite growth.
• The protein localizes to both the nucleus and cytoplasm.
• It is broadly expressed in tissues including brain, heart, muscle, placenta, lung, liver, kidney, and pancreas.
• SMARCC1 is subject to multiple post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination, and is associated with neurogenesis and disease variants.

Experimental Guidance and Technical Tips

• This antibody was generated using a recombinant protein corresponding to amino acids 700-800 of human SMARCC1/BAF155, which may provide specific recognition of this region.
• In Western blot (WB), a predominant band at ~123 kDa is expected; consider using appropriate loading controls and validate in your sample system.
• For immunofluorescence (IF/ICC), anticipate nuclear and potentially cytoplasmic staining; fixation and permeabilization conditions should be optimized.
• Chromatin immunoprecipitation (ChIP) may be feasible; however, due to the large size of the SWI/SNF complex, it may require careful optimization of crosslinking and shearing protocols.
• Cross-reactivity is reported for human and mouse; for other species, sequence homology should be checked.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw material partner, offering diagnostic manufacturers and research laboratories access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets. For chromatin remodeling and gene regulation studies, our anti-SMARCC1/BAF155 antibody exemplifies the quality materials we provide to accelerate your assay development from concept to clinic.