Anti-SMARCB1/SNF5 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q12824

Core Product Specifications and Parameters

Biological Background: SMARCB1/SNF5 Function and Localization

• Core component of the BAF (hSWI/SNF) ATP-dependent chromatin-remodeling complex, which regulates gene expression by altering nucleosome structure.
• Stimulates the chromatin remodeling activity of SMARCA4/BRG1 and is involved in activating the CSF1 promoter.
• Plays a critical role in cell-cycle control, inducing G0/G1 arrest; functions as a tumor suppressor frequently inactivated in malignant rhabdoid tumors.
• Essential for the self-renewal capacity of neural stem/progenitor cells as part of the npBAF complex, and for postmitotic neuron dendritic growth via the nBAF complex.
• Localizes to the nucleus, consistent with its role in chromatin dynamics and transcription regulation.
• Undergoes post-translational modifications including phosphorylation, isopeptide bond formation, and ubiquitination, which may modulate its activity and stability.
• Multiple isoforms are produced by alternative splicing, expanding functional diversity.

Experimental Guidance and Technical Tips

• The immunogen used to generate this polyclonal antibody is a synthetic peptide spanning residues 50–150 of human SMARCB1/SNF5. Consider epitope location when designing experiments.
• For Western blotting, a band around 44 kDa is expected; validate detection in relevant human, mouse, or rat cell lysates.
• Recommended applications include WB, IHC-P, IF/ICC, IP, and ELISA. Optimize antibody dilution and incubation conditions for each assay.
• As a polyclonal antibody, lot-to-lot consistency should be verified in your specific application system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing platform for IVD raw materials, supporting diagnostic developers and research institutions from concept to commercialization. This anti-SMARCB1/SNF5 polyclonal antibody exemplifies our commitment to providing high-quality reagents for chromatin biology and oncology research. Beyond individual antibodies, we supply validated antibody pairs, bulk ancillary reagents, and rare-target sourcing, backed by technical consultation to streamline your assay development.