Anti-SMARCB1/SNF5 Polyclonal Antibody for WB, ELISA - Q12824

Core Product Specifications and Parameters

Biological Background: SMARCB1/SNF5 Function and Localization

• SMARCB1/SNF5, also known as BAF47, INI1, or hSNF5, is a core subunit of the SWI/SNF (BAF) ATP-dependent chromatin remodeling complex, critical for regulating gene expression through nucleosome remodeling.
• The BAF complex modulates chromatin structure by creating altosomes—asymmetric structures containing two histone octamers—thereby altering DNA supercoiling and facilitating transcriptional regulation, with SMARCB1/SNF5 stimulating the remodeling activity of SMARCA4/BRG1.
• SMARCB1/SNF5 is involved in diverse cellular processes including cell proliferation and differentiation, antiviral responses, and tumor suppression; it enforces G0/G1 cell cycle arrest and functions as a tumor suppressor frequently inactivated in malignant rhabdoid tumors.
• During neural development, SMARCB1/SNF5 is a component of both neural progenitor (npBAF) and neuron-specific (nBAF) complexes, where subunit switching (e.g., ACTL6A/B and PHF10/DPF1/3) drives the transition from stem cell self-renewal to post-mitotic neuronal differentiation.
• The protein localizes exclusively to the nucleus, as indicated by its role in chromatin binding and transcriptional regulation.
• Post-translational modifications include phosphorylation and ubiquitination (isopeptide bond formation), and SMARCB1/SNF5 is known to interact with viral proteins (e.g., HIV-1 integrase), linking it to host-virus interactions.
• Mutations in SMARCB1 are associated with Coffin-Siris syndrome (intellectual disability, hypotrichosis) and schwannomatosis, underscoring its importance in developmental and tumorigenic pathways.

Experimental Guidance and Technical Tips

• The immunogen for this antibody corresponds to amino acids 50–150 of human SMARCB1/SNF5 (NP_003064.2). This region is well-conserved across species, consistent with the observed reactivity with mouse and rat orthologs.
• For Western blotting, a band at approximately 44 kDa is expected. We recommend titrating the antibody and including appropriate positive controls (e.g., HeLa, HEK293, or mouse/rat brain lysates) to confirm specificity.
• ELISA applications may benefit from using recombinant SMARCB1/SNF5 protein or peptide as coating antigen. The antibody may also detect endogenous SMARCB1/SNF5 in cell lysates, though optimization of coating conditions is advised.
• Consider verifying knockdown or knockout cell lines to control for specificity in immunodetection assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials centered on chromatin biology and oncology targets like SMARCB1/SNF5. From validated antibody pairs and optimized polyclonal antibodies to bulk recombinant proteins and ancillary reagents, we streamline procurement of high-quality components. Whether you require rare-target sourcing or scale-up support, CamelBio bridges your workflow—from concept to clinic—with reliable, one-stop access to critical raw materials.