Anti-SMARCB1/SNF5 Monoclonal Antibody for WB, IF/ICC, ELISA - Q12824

Core Product Specifications and Parameters

Biological Background: SMARCB1/SNF5 Function and Localization

• SMARCB1/SNF5 (also known as BAF47, INI1, hSNF5) is a core subunit of the ATP-dependent BAF (SWI/SNF) chromatin remodeling complex, which modulates nucleosome structure and DNA accessibility.
• The BAF complex converts up to half of nucleosomes into asymmetric altosomes, each containing two histone octamers, thereby altering DNA supercoiling and facilitating transcriptional regulation.
• SMARCB1 directly stimulates the remodeling activity of the SMARCA4/BRG1 ATPase and is required for activation of the CSF1 promoter, linking it to macrophage and osteoclast differentiation.
• It is an integral component of both npBAF (neural progenitor) and nBAF (neuron-specific) chromatin remodeling complexes, with subunit switching during neuronal differentiation essential for proper dendrite morphogenesis and postmitotic neuronal identity.
• SMARCB1 functions as a tumor suppressor, as evidenced by its role in cell cycle control — it causes G0/G1 arrest and is frequently inactivated in malignant rhabdoid tumors and other cancers.
• The protein is localized exclusively to the nucleus, consistent with its role in chromatin regulation and transcription.
• Key post-translational modifications include phosphorylation (phosphoprotein) and ubiquitination (Ubl conjugation), which may regulate its stability and interactions.
• Alternative splicing generates multiple isoforms, expanding functional diversity; it also participates in host-virus interactions (e.g., HIV-1 integrase binding).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 275-375) of human SMARCB1, which is well-conserved across human, mouse, and rat (based on specified cross-reactivity). This region is not within known functional domains, potentially reducing interference with protein interactions.
• For Western blot (WB), the predicted molecular weight is ~44 kDa. Consider using whole-cell lysates from human, mouse, or rat cell lines; include appropriate controls (e.g., SMARCB1 knockout lysates if available) to confirm band specificity.
• For immunofluorescence/immunocytochemistry (IF/ICC), expect nuclear staining. Permeabilization and fixation conditions should be optimized; co-staining with nuclear markers (e.g., DAPI) is recommended.
• In ELISA applications, the antibody may be used for direct or sandwich formats; validate sensitivity and specificity with recombinant SMARCB1 protein or cell lysates.
• Given the critical role of SMARCB1 in chromatin remodeling and tumor suppression, this antibody is suitable for studying chromatin biology, neurodevelopment, and cancer mechanisms. Validate in the relevant sample system and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For chromatin remodeling and oncology research, we provide high-quality antibodies like the SMARCB1/SNF5 Rabbit mAb to support diagnostic assay development. Our team also assists with rare target raw-material sourcing and technical consulting, helping you accelerate from concept to clinic.