Anti-SMARCAD1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H4L7

Core Product Specifications and Parameters

Biological Background: SMARCAD1 Function and Localization

• SMARCAD1 is an ATP-dependent chromatin remodeler belonging to the SWI/SNF subfamily, possessing both nucleosome-remodeling and histone chaperone activities. Related references: PMID:21820097

• It plays a critical role in DNA double-strand break repair by promoting DNA end resection, likely through weakening histone–DNA interactions at damage sites. Related references: PMID:22960744

• SMARCAD1 is essential for the restoration of heterochromatin organization after replication, facilitating histone deacetylation and H3K9me3 modification. Related references: PMID:21549307

• The protein localizes to the nucleus and associates with chromosomes, consistent with its functions in chromatin dynamics.

• Isoform 1 is ubiquitously expressed, while isoform 3 is predominantly expressed in skin and esophagus; both have been detected in fibroblasts and keratinocytes.

• Key post-translational modifications include acetylation, phosphorylation, and isopeptide bond formation, which may regulate its activity.

• Mutations in SMARCAD1 are associated with ectodermal dysplasia and palmoplantar keratoderma, highlighting its role in skin development.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–300) of human SMARCAD1, which may help detect both major isoforms; however, isoform-specific validation is advised.
• For western blot, the predicted molecular weight is 117 kDa; ensure appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• For IF/ICC, permeabilization and fixation conditions should be optimized for nuclear staining; include appropriate controls to confirm specificity.
• As a polyclonal antibody, lot-to-lot variability should be considered; validation in the relevant sample system is recommended before critical experiments.

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