Anti-SMARCAD1 Polyclonal Antibody for WB, ELISA - Q9H4L7

Core Product Specifications and Parameters

Biological Background: SMARCAD1 Function and Localization

• SMARCAD1 (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1) is an ATP-dependent chromatin remodeler with intrinsic nucleosome-remodeling activity, playing essential roles in DNA repair and heterochromatin organization. Related references: PMID:22960744, PMID:21820097
• The protein combines ATP-dependent histone exchange with chaperone-like ATP-independent histone deposition and nucleosome assembly, facilitating chromatin dynamics. Related references: PMID:21820097
• SMARCAD1 promotes DNA end resection at double-strand breaks (DSBs) by weakening histone-DNA interactions in flanking nucleosomes, thereby supporting DNA repair. Related references: PMID:22960744
• It is required for the restoration of heterochromatin organization after replication, acting at replication sites to direct H3 and H4 deacetylation, H3K9me3 trimethylation, and restoration of silencing. Related references: PMID:21549307
• Subcellular localization is predominantly nuclear, associated with chromosomes, consistent with its role in chromatin regulation.
• Isoform 1 is expressed ubiquitously, while isoform 3 is mainly expressed in skin and esophagus; SMARCAD1 is detected at the protein level in fibroblasts and keratinocytes.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination; it is annotated with keywords such as ATP-binding, DNA-binding, chromatin regulator, and hydrolase.

Experimental Guidance and Technical Tips

• For Western blotting, expect bands around 68 kDa and/or 117 kDa corresponding to different isoforms; verify specificity using appropriate positive and negative controls.
• In ELISA, the immunogen region (aa 928–1026) can serve as a positive antigen control; optimize coating concentrations and detection conditions.
• Use nuclear extracts or whole-cell lysates from human cell lines for target detection; cross-reactivity with other species has not been tested.
• Consider pairing with a secondary anti-rabbit IgG antibody and validating in the relevant experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, technical services, and consulting from concept to clinic. For chromatin biology and DNA repair research, we supply validated antibody pairs, optimized polyclonal antibodies like this anti-SMARCAD1, bulk ancillary reagents, and rare target sourcing to support your diagnostic or research workflows. Let us streamline your procurement with reliable, one-stop solutions tailored to your needs.