Anti-SMARCA5/SNF2H Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - O60264

Core Product Specifications and Parameters

Biological Background: SMARCA5/SNF2H Function and Localization

• SMARCA5, also known as SNF2H or ISWI, is the catalytic subunit of ISWI chromatin-remodeling complexes with intrinsic ATP-dependent nucleosome-remodeling activity. Related references: PMID:12972596, PMID:28801535
• It forms complexes with BAZ1A/ACF1, BAZ1B/WSTF, BAZ2A/TIP5, BAZ2B, CECR2, and RSF1 to regulate nucleosome spacing and sliding, and is essential for assembling regular nucleosome arrays. Related references: PMID:12972596, PMID:14759371, PMID:15543136, PMID:28801535
• SMARCA5 is required for DNA replication of pericentric heterochromatin in S-phase, in conjunction with BAZ1A/ACF1. Related references: PMID:12434153
• It participates in RNA polymerase I and III transcription through the WICH and B-WICH ISWI chromatin-remodeling complexes. Related references: PMID:11980720, PMID:15543136, PMID:16603771
• Subcellularly, SMARCA5 localizes to the nucleus and chromosomes.
• Ubiquitously expressed, it undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitination.
• SMARCA5 mediates histone H2AX phosphorylation at Tyr-142 and maintains chromatin structure during DNA replication.
• Additional associated keywords: 3D-structure, host-virus interaction, isopeptide bond, repeat, reference proteome.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 953-1052); consider this for epitope specificity in applications.
• For Western blot (WB), detect SMARCA5 at approximately 122 kDa; validate with appropriate positive controls.
• In immunofluorescence (IF/ICC), nuclear permeabilization is recommended due to its chromatin localization.
• For ELISA, optimize coating and detection conditions based on the target antigen.
• Cross-reactivity with mouse and rat allows for versatile model organism studies; validate in the relevant species tissue or cell line.
• Pair with appropriate secondary antibodies and detection reagents for consistent results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, including this anti-SMARCA5/SNF2H monoclonal antibody, to support your chromatin remodeling and epigenetics research. Beyond validated antibodies, we offer bulk reagent procurement, custom antibody development, and rare target sourcing to accelerate your diagnostic and R&D projects from concept to clinic.