Anti-SMARCA2 / BRM Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ChIP, ELISA - P51531

Core Product Specifications and Parameters

Biological Background: SMARCA2/BRM Function and Localization

• SMARCA2 (BRM) is a DNA-binding ATPase that acts as a transcription regulator by altering nucleosome structure and controlling chromatin accessibility. Related references: PMID:15075294 PMID:22952240 PMID:26601204
• The protein is a core catalytic subunit of the SWI/SNF chromatin remodeling complexes, which use ATP hydrolysis to mobilize nucleosomes and regulate gene expression genome-wide.
• SMARCA2 is specifically assembled into neural progenitor-specific (npBAF) and neuron-specific (nBAF) complexes, playing an essential role in the self-renewal of neural stem cells and the subsequent differentiation into postmitotic neurons.
• Subcellularly, SMARCA2 localizes to the nucleus, consistent with its role in chromatin-based transcriptional regulation.
• The protein contains a bromodomain that recognizes acetylated histones, an ATPase domain essential for its remodeler activity, and is subject to post-translational modifications such as acetylation and phosphorylation.
• Mutations in SMARCA2 are associated with developmental disorders such as Nicolaides-Baraitser syndrome (NCBRS), featuring intellectual disability and hypotrichosis.
• Additional keyword annotations include roles in neurogenesis, alternative splicing, ubiquitin conjugation, and potential involvement in schizophrenia, highlighting the broad biological significance of this protein.

Experimental Guidance and Technical Tips

• This antibody is raised against a synthetic peptide. It may recognize denatured SMARCA2 in Western blotting (WB) and may also work in applications like IHC-P, IF/ICC, IP, ChIP, and ELISA; however, individual optimization is recommended.
• SMARCA2 is a large protein with a predicted molecular weight of 181 kDa. Use low-percentage SDS-PAGE gels (e.g., 6-8%) and ensure adequate transfer conditions for high-molecular-weight proteins.
• For chromatin immunoprecipitation (ChIP), carefully optimize fixation and chromatin shearing, as SMARCA2 is a nuclear chromatin-associated factor.
• In immunofluorescence (IF/ICC), expect predominant nuclear staining, and include appropriate controls to verify specificity.
• Cross-reactivity is documented for human; endogenous levels may vary across cell lines and tissues. Validate dilutions and conditions in your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing one-stop access to high-quality IVD raw materials, including validated monoclonal antibodies, antibody pairs, and ancillary reagents. For researchers focused on chromatin remodeling, neurodevelopment, or transcriptional regulation, we offer reliable sourcing of SMARCA2/BRM antibodies and related critical raw materials to support your assay development from concept to clinic.