Anti-SMARCA1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P28370

Core Product Specifications and Parameters

Biological Background: SMARCA1 Function and Localization

• SMARCA1 encodes the catalytic subunit of ISWI chromatin-remodeling complexes, with intrinsic ATP-dependent activity that is elevated on nucleosome substrates. Related references: PMID:14609955, PMID:15310751, PMID:15640247
• Depending on associated subunits, ISWI complexes containing SMARCA1 can slide mononucleosomes to the center (BAZ1A/B, BAZ2A/B) or lack this activity (CECR2, RSF1). Related references: PMID:28801535
• SMARCA1 facilitates histone octamer assembly onto nascent DNA during replication, preserving fork progression and preventing DNA gaps. Related references: PMID:39413208
• In the NURF-1 complex, SMARCA1 positively regulates En1 and En2 expression, contributing to brain development and neurite outgrowth. Related references: PMID:14609955
• An alternatively spliced, catalytically inactive isoform acts as a dominant-negative regulator of chromatin remodeling. Related references: PMID:15310751, PMID:28801535
• Subcellular localization is nuclear, associated with chromosomes.
• Expressed predominantly in non-neuronal tissues (lung, breast, kidney, ovary) with additional expression in skeletal muscle and brain.
• Post-translational modifications include phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal 200 amino acids, suggesting the antibody may recognize multiple isoforms containing this region. Verify reactivity in your sample system.
• For Western blot, a band near 123 kDa is expected; additional bands from isoforms or PTMs may appear.
• In immunohistochemistry, consider antigen retrieval and positive control tissues such as lung or breast.
• For immunofluorescence, nuclear localization should be observed; co-staining with DNA dyes is recommended.
• For immunoprecipitation, use standard lysis conditions; elution with low pH or SDS buffer may be necessary.
• ELISA can be employed for quantitative detection; optimize coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supporting your chromatin remodeling and epigenetic research needs. We supply a comprehensive portfolio of validated antibodies, including this SMARCA1 polyclonal antibody, alongside optimized monoclonal/polyclonal pairs, bulk ancillary reagents, and rare target sourcing. Contact us to streamline your diagnostic development from concept to clinic.