Anti-SMAP1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8IYB5

Core Product Specifications and Parameters

Biological Background: SMAP1 Function and Localization

• SMAP1 (Stromal membrane-associated protein 1) is a GTPase-activating protein (GAP) that specifically acts on ARF6, a small GTPase.
• It plays a role in clathrin-dependent endocytosis, regulating membrane trafficking.
• By similarity, SMAP1 may also be involved in erythropoiesis.
• The protein localizes to the cell membrane.
• SMAP1 is expressed in multiple tissues including bone marrow, adrenal gland, trachea, lymph node, spinal cord, peripheral blood leukocytes, thyroid, and stomach.
• The protein contains a zinc-finger domain and binds zinc, and undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 180-250 of human SMAP1; consider validating detection in relevant cell or tissue lysates.
• For Western blot, a band around 50 kDa is predicted; confirm specificity using appropriate positive and negative controls (e.g., SMAP1-overexpressing cells or siRNA knockdown).
• In immunofluorescence/ICC, optimize fixation and permeabilization conditions to preserve membrane localization.
• Cross-reactivity with mouse and rat SMAP1 is reported; verify sequence homology in the target region before use in these species.
• ELISA applications may require optimization of coating antigen and antibody dilution; use recombinant protein as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibodies like this anti-SMAP1 polyclonal antibody, to support your endocytosis and erythropoiesis research. We provide one-stop access to optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing services for rare targets, streamlining your assay development from concept to clinic.