Anti-Smad5 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q99717

Core Product Specifications and Parameters

Biological Background: Smad5 Function and Localization

• SMAD5 is a member of the SMAD family, also known as JV5-1, Mothers against decapentaplegic homolog 5 (MAD homolog 5), encoded by the SMAD5 (MADH5) gene.
• Upon BMP ligand stimulation, SMAD5 is phosphorylated by activated type I BMP receptors (BMPRIs) and forms a complex with SMAD4. This complex translocates to the nucleus and regulates gene transcription by binding to Smad Binding Elements (SBEs) (PubMed:9442019, PubMed:33510867). Related references: PMID:9442019 PMID:33510867
• SMAD5 plays a role in various biological processes including embryonic development, cell differentiation, angiogenesis, and tissue homeostasis (PubMed:12064918, PubMed:16516194). Related references: PMID:12064918 PMID:16516194
• In its non-phosphorylated state, SMAD5 localizes to the cytoplasm and mitochondria, where it promotes mitochondrial respiration and glycolysis by interacting with hexokinase 1 (HK1) in response to cytoplasmic pH changes (PubMed:28675158). Related references: PMID:28675158
• Subcellular localization includes the cytoplasm, nucleus, and mitochondrion, reflecting its dual role in signaling transduction and energy metabolism.
• Ubiquitously expressed across various tissues.
• Post-translationally modified by phosphorylation and acetylation, and contains zinc-binding and DNA-binding domains essential for its function as a transcriptional regulator.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band around 52 kDa; use appropriate loading controls and validate with known positive and negative cell lysates.
• In immunohistochemistry (IHC-P), antigen retrieval may be required due to nuclear/cytoplasmic localization; test on tissue sections with confirmed SMAD5 expression.
• The immunogen spans the C-terminal region (amino acids 186-465) including the MH2 domain, thus the antibody may recognize both phosphorylated and unphosphorylated forms; confirm activation states using phospho-specific antibodies or treatments.
• Cross-reactivity with mouse and rat indicates conserved epitopes; when applying to other species, check sequence homology and perform validation.
• For ELISA, optimize coating conditions and use recombinant SMAD5 or cell lysates as positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with a comprehensive portfolio of IVD raw materials, including this high-quality anti-SMAD5 polyclonal antibody for BMP signaling research and assay development. Beyond antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and expert assistance in sourcing rare-target raw materials to streamline your workflow from concept to clinic.