Anti-Smad3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P84022

Core Product Specifications and Parameters

Biological Background: Smad3 Function and Localization

• SMAD family member 3 (Smad3), also known as MAD homolog 3 or hMAD-3, is an intracellular signal transducer and transcriptional modulator encoded by the SMAD3 (MADH3) gene.
• As a receptor-regulated SMAD (R-SMAD), Smad3 mediates TGF-beta and activin signaling by binding to type 1 receptor kinases.
• Upon phosphorylation, Smad3 forms a complex with SMAD4, translocates to the nucleus, and binds the TRE element in promoter regions to regulate transcription of target genes.
• It can also interact with the AP-1 transcription factors JUN/FOS to form a complex at AP-1/SMAD sites, thereby modulating transcription.
• Smad3 shuttles between the cytoplasm and nucleus, with its subcellular localization dynamically influenced by signaling activity.
• The protein undergoes multiple post-translational modifications, including phosphorylation, acetylation, ADP-ribosylation, and ubiquitination, and contains a zinc-binding domain.
• Alternative splicing generates multiple isoforms, and Smad3 is implicated in processes such as wound healing, chondrogenesis, osteogenesis, and aortic aneurysm.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 150–250 of human Smad3, a region expected to be exposed and conserved across human, mouse, and rat, supporting cross-reactivity.
• For Western blot, expect a band around 48 kDa; validate detection in relevant cell lysates and consider appropriate controls to confirm specificity.
• In immunofluorescence/ICC, optimize fixation and permeabilization conditions, and use nuclear counterstaining to monitor Smad3 translocation upon TGF-beta stimulation.
• For ELISA, pair with a matched detection antibody and test serial dilutions of recombinant Smad3 or native samples to establish linear range and sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a streamlined, one-stop sourcing solution for IVD raw materials. This Smad3 antibody supports investigations into TGF-beta signaling and transcriptional regulation, and we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your development from concept to clinic.