Anti-Smad3 Rabbit Monoclonal Antibody (KO Validated) for WB, IHC-P, ELISA - P84022

Core Product Specifications and Parameters

Biological Background: Smad3 Function and Localization

• SMAD family member 3 (Smad3) is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases.
• It binds to the TRE element in the promoter region of many TGF-beta-regulated genes and, upon forming the SMAD3/SMAD4 complex, activates transcription. It can also assemble into a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription.
• Smad3 negatively regulates wound healing, likely by modulating both growth and migration of primary keratinocytes and by altering TGF-beta-mediated chemotaxis of monocytes. This effect appears to be hormone-sensitive.
• It serves as a regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures.
• Smad3 positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ, which acts as a negative regulator.
• The protein localizes to both the cytoplasm and the nucleus.
• Post-translational modifications include acetylation, ADP-ribosylation, phosphorylation, and ubiquitination. It contains metal-binding and DNA-binding domains and participates in host-virus interactions.
• Alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to amino acids 100–200 of human Smad3 (NP_005893.1). When applying to non-human species, confirm sequence homology to ensure reactivity.
• For Western blot (WB), use appropriate loading controls and verify the band at approximately 48 kDa; knockout validation is recommended to confirm specificity in your samples.
• For IHC-P, optimize antigen retrieval conditions and include both positive and negative tissue controls to validate staining patterns.
• For ELISA applications, consider pairing with a suitable capture or detection antibody for sandwich formats, and confirm performance using recombinant Smad3 protein.
• Cross-reactivity with mouse and rat has been validated; however, independent verification in your specific experimental system is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets. Whether you need anti-Smad3 antibodies for TGF-beta signaling research or other components, we provide one-stop procurement support from concept to clinic. Contact us to accelerate your diagnostic development with reliable, batch-tested reagents.