Anti-Smad3 (KO Validated) Monoclonal Antibody for WB, IP, IHC-P, ELISA - P84022

Core Product Specifications and Parameters

Biological Background: Smad3 Function and Localization

• SMAD3 (Mothers against decapentaplegic homolog 3, also known as MADH3, hMAD-3, JV15-2) is an intracellular signal transducer and transcriptional modulator belonging to the SMAD family.
• It functions as a receptor-regulated SMAD (R-SMAD) activated by TGF-beta and activin type 1 receptor kinases, binding to the TRE element in target gene promoters.
• Upon phosphorylation, SMAD3 forms a complex with SMAD4 and translocates to the nucleus to regulate transcription of TGF-beta-responsive genes; it can also interact with JUN/FOS at AP-1/SMAD sites.
• SMAD3 exerts an inhibitory effect on wound healing by modulating keratinocyte growth, migration, and monocyte chemotaxis in a hormone-sensitive manner.
• It acts as a key regulator of chondrogenesis and osteogenesis, inhibiting early bone fracture healing.
• SMAD3 positively regulates PDPK1 kinase activity by stimulating its dissociation from the inhibitory 14-3-3 protein YWHAQ.
• Subcellular localization is dynamic: predominantly cytoplasmic under basal conditions but accumulates in the nucleus upon pathway activation.
• Post-translational modifications include phosphorylation, acetylation, ADP-ribosylation, and ubiquitination, with zinc binding being essential for function; it is implicated in aortic aneurysm and viral interactions.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein spanning amino acids 119-219 of human Smad3, which may confer high specificity; consider verifying performance in your specific experimental context.
• This antibody is KO validated, making it suitable for Western blot (WB) application to assess specificity via knockout cell lines.
• For immunoprecipitation (IP) and immunohistochemistry (IHC-P), confirm compatibility with your sample preparation and detection protocols.
• ELISA can be employed for quantitative analysis; include appropriate controls and validate against known standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes streamlined access to premium IVD raw materials. This anti-Smad3 monoclonal antibody is part of our comprehensive portfolio for TGF-beta signaling and transcription regulation research. In addition to validated antibodies, we can source matched antibody pairs, bulk ancillary reagents, and provide consultation on integrating raw materials into your assay development workflow.