Anti-Smad1 Polyclonal Antibody for WB, ELISA - Q15797

Core Product Specifications and Parameters

Biological Background: Smad1 Function and Localization

• SMAD1 acts as a transcriptional modulator, playing pivotal roles in embryonic development, cell differentiation, and tissue homeostasis (PubMed:9335504). Related references: PMID:9335504

• Upon BMP ligand stimulation, SMAD1 is phosphorylated by activated type I BMP receptors, associates with SMAD4, and the heteromeric complex translocates to the nucleus to bind Smad Binding Elements (SBEs) and regulate target gene expression (PubMed:33667543). Related references: PMID:33667543

• SMAD1 localizes to both the cytoplasm and nucleus, with nuclear translocation occurring upon BMP signaling activation.

• The protein is expressed ubiquitously, with the highest levels observed in heart and skeletal muscle.

• Post-translational modifications include phosphorylation, acetylation, and ubiquitination; it also contains a zinc-binding MH1 domain.

• SMAD1 positively regulates BMP4-induced MSX1 expression, a key factor in odontogenic development (by similarity).

Experimental Guidance and Technical Tips

• For western blot (WB), this polyclonal antibody can detect endogenous Smad1 in human, mouse, and rat samples; consider using a suitable blocking buffer and membrane to minimize background.
• In ELISA, the antibody can be paired with appropriate antigens; validate the assay conditions, including coating buffers and detection antibodies.
• The immunogen corresponds to a highly conserved region (aa 20–240), suggesting reactivity across multiple species and possibly multiple isoforms.
• For functional studies, combining with phospho-specific antibodies may help distinguish total vs. activated SMAD1.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with high-quality raw materials like this anti-Smad1 polyclonal antibody, covering BMP signaling and developmental biology applications. We offer one-stop access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your IVD development from concept to clinic.