Anti-Smad1 Monoclonal Antibody (KO Validated) for WB, IF/ICC, ELISA - Q15797

Core Product Specifications and Parameters

Biological Background: Smad1 Function and Localization

• Smad1 is a member of the SMAD family of transcriptional modulators that mediate BMP signaling pathways, playing critical roles in embryonic development, cell differentiation, and tissue homeostasis. Related references: PMID:9335504
• Upon BMP receptor activation, Smad1 is phosphorylated by type I BMP receptors (BMPRIs) and forms a complex with SMAD4, which translocates to the nucleus to regulate target gene expression. Related references: PMID:33667543
• The SMAD1/SMAD4 complex recognizes Smad Binding Elements (SBEs) on DNA, thereby modulating transcriptional outcomes in response to BMP signals. Related references: PMID:33667543
• Smad1 is subject to post-translational modifications including phosphorylation and acetylation, which regulate its activity and stability.
• Subcellular localization includes both the cytoplasm and nucleus, consistent with its role as a signal transducer and transcription factor.
• Smad1 is ubiquitously expressed, with highest expression levels observed in the heart and skeletal muscle.
• The gene encoding Smad1 undergoes alternative splicing, generating multiple isoforms that may have distinct functions.
• Keywords associated with Smad1 include DNA-binding, metal-binding (zinc), phosphoprotein, and Ubl conjugation, reflecting its diverse regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 160-260 of human Smad1; consider this region when validating antibody specificity in your assay system.
• For Western blot (WB), the predicted molecular weight of Smad1 is approximately 52 kDa; an appropriate molecular weight marker range should be included.
• Immunofluorescence (IF/ICC) applications may require optimization of fixation and permeabilization conditions to preserve the nuclear and cytoplasmic localization of Smad1.
• For ELISA, it is advisable to validate the antibody in your specific sandwich or indirect ELISA setup, including appropriate controls and reagent pairings.
• Since this antibody has been KO validated, consider including knockout cell lines or tissues as negative controls in WB and IF/ICC experiments to confirm specificity.
• Cross-reactivity with mouse Smad1 has been confirmed; however, when working with other species, verify sequence homology and antibody binding by experimental validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies your raw-material procurement by offering a comprehensive portfolio of validated IVD antibodies, including this Smad1 monoclonal antibody for developmental biology and signaling research. As a one-stop sourcing partner, we provide optimized antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, ensuring your projects move seamlessly from concept to clinic. Leverage our expertise to accelerate your diagnostic development with reliable, ready-to-scale materials.