Anti-Smad1 Monoclonal Antibody for WB, IHC-P, IP, ELISA - Q15797

Core Product Specifications and Parameters

Biological Background: Smad1 Function and Localization

• Transcriptional modulator involved in embryonic development, cell differentiation, and tissue homeostasis. Upon BMP ligand binding, SMAD1 is phosphorylated by type I BMP receptors, forms a complex with SMAD4, and translocates to the nucleus to regulate gene expression by binding Smad Binding Elements (SBEs). Related references: PMID:9335504, PMID:33667543
• Part of the SMAD family, acting as a critical intracellular mediator of the BMP signaling pathway, with key roles in odontogenesis and skeletal development.
• SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1, modulating transcriptional coactivator availability.
• Positively regulates BMP4-induced expression of the odontogenic regulator MSX1 following IPO7-mediated nuclear import.
• Shuttles between the cytoplasm and nucleus; undergoes nuclear accumulation upon pathway activation.
• Ubiquitously expressed, with highest levels observed in the heart and skeletal muscle.
• Subject to multiple post-translational modifications including phosphorylation, acetylation, and ubiquitination; contains a zinc-binding MH1 domain.
• Alternative splicing produces multiple protein isoforms, expanding functional diversity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 20–240 of human Smad1, encompassing the conserved MH1 domain; cross-reactivity with mouse Smad1 is supported by sequence homology.
• For Western blot analysis, a band of approximately 52 kDa is expected. Include phosphatase inhibitors in lysis buffer to preserve phosphorylation-dependent signals.
• Immunoprecipitation and ELISA applications have been reported; optimal antibody concentration should be empirically determined for each assay.
• For immunohistochemistry (paraffin), antigen retrieval may be necessary; heart and skeletal muscle sections serve as positive control tissues.
• Consider the nuclear-cytoplasmic shuttling of Smad1 when interpreting localization data; BMP stimulation may alter subcellular distribution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive IVD raw-material sourcing, including validated monoclonal antibodies like this Smad1 product for BMP/TGF-β pathway studies. Beyond catalog antibodies, we offer one-stop access to antibody pairs, ancillary reagents, and custom rare-target procurement—streamlining your development from concept to clinic.