Anti-Smac/Diablo Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q9NR28

Core Product Specifications and Parameters

Biological Background: Smac/Diablo Function and Localization

• Diablo IAP-binding mitochondrial protein, also known as Second mitochondria-derived activator of caspases (SMAC), is encoded by the DIABLO gene and plays a central role in apoptotic signaling.
• It promotes apoptosis by neutralizing inhibitor of apoptosis proteins (IAPs) such as XIAP/BIRC4 and BIRC6/BRUCE, thereby enabling caspase-9 and caspase-3 activation.
  Related references: PMID:15200957, PMID:36758104, PMID:36758105
• Normally localized to the mitochondrial intermembrane space; upon apoptotic stimuli, it is released into the cytosol to execute its pro-apoptotic function.
• Ubiquitously expressed with highest levels in testis, and also abundant in heart, liver, kidney, spleen, prostate, and ovary; lower expression in brain, lung, thymus, and peripheral blood leukocytes.
• Alternative splicing generates multiple isoforms; isoform 3 is widely expressed across tissues.
• Keywords associated with this protein include apoptosis, deafness (DFNA64), alternative splicing, and ubiquitin-like conjugation, reflecting its diverse regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 56–239) corresponds to the mature Smac/Diablo protein lacking the mitochondrial targeting sequence; for Western blot, expect a band around 27 kDa (processed form), and consider enriching mitochondrial fractions or using apoptosis-induced lysates for optimal detection.
• As a polyclonal antibody, it may recognize multiple epitopes, offering robust signal in immunoprecipitation (IP) for capturing Smac/Diablo complexes; validate in your specific sample system.
• For immunohistochemistry (IHC-P), test on tissues with high expression such as testis, and include appropriate controls to confirm staining specificity.
• Cross-reactivity with mouse and rat is indicated; verify sequence homology and perform experimental validation when working with these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this rabbit polyclonal anti-Smac/Diablo antibody as part of a comprehensive IVD raw-materials portfolio supporting apoptosis research and diagnostic assay development. Beyond primary antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and custom sourcing for rare targets—ensuring a seamless transition from early development to clinical application.