Anti-Slug Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O43623

Core Product Specifications and Parameters

Biological Background: Slug Function and Localization

• Slug (SNAI2) is a zinc finger transcriptional repressor that modulates both activator-dependent and basal transcription, belonging to the Snail family of transcription factors.
• It plays a critical role in epithelial-mesenchymal transition (EMT) by repressing E-cadherin (CDH1) transcription via E-box elements, promoting cell invasion and metastasis.
• Slug is involved in the generation and migration of neural crest cells during development, highlighting its role in embryogenesis.
• The protein binds to specific promoters (e.g., BRCA2, ITGA3, BSG) to regulate gene expression in contexts such as breast cancer, keratinocyte adhesion, and hepatocyte EMT.
• Slug participates in osteoblast maturation by binding to RUNX2 and SOC9 promoters, acting as both a positive and negative regulator.
• Subcellular localization includes the nucleus and cytoplasm, consistent with its transcriptional regulatory functions and potential shuttling.
• Slug is widely expressed in adult tissues (spleen, thymus, prostate, testis, ovary, intestine, heart, brain, lung, liver, kidney, etc.) and shows elevated expression in basal epidermal layers, osteoblasts, mesenchymal stem cells, and breast tumor cells.
• Post-translational modifications include ubiquitination, and the protein is implicated in Waardenburg syndrome and deafness when mutated.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 21–120 of human Slug (NP_003059.1); consider potential cross-reactivity with other Snail family members and validate specificity in your model system.
• For Western blotting, Slug has a predicted molecular weight of ~30 kDa; ensure appropriate gel percentage and transfer conditions for low molecular weight proteins.
• In immunohistochemistry (IHC-P), antigen retrieval may be required; optimize staining using known positive and negative tissue controls.
• ELISA applications may benefit from pairing this polyclonal antibody with a monoclonal capture antibody; consider titration for optimal signal-to-noise ratio.
• Sample preparation (e.g., subcellular fractionation) can help enrich nuclear Slug for WB or IP due to its nucleocytoplasmic distribution.
• Always include relevant controls (e.g., siRNA knockdown, overexpression lysates) to confirm band identity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. The Slug antibody supports research in EMT, cancer metastasis, and developmental biology, areas where reliable detection reagents are critical. We supply validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare-target raw materials to streamline your IVD development pipeline.