Anti-Slug Rabbit Monoclonal Antibody for WB and ELISA - O43623

Core Product Specifications and Parameters

Biological Background: Slug Function and Localization

• Slug (SNAI2) is a zinc finger transcriptional repressor of the Snail family, containing DNA-binding zinc-finger domains.
• It modulates both activator-dependent and basal transcription, playing critical roles in neural crest cell generation and migration during development.
• Slug represses E-cadherin/CDH1 transcription via E-box elements, acting as a key inducer of epithelial-mesenchymal transition (EMT) and promoting invasion and metastasis.
• In osteoblasts, Slug binds to RUNX2 and SOC9 promoters, functioning as both a positive and negative transcriptional regulator during osteoblast maturation.
• The protein localizes to both the nucleus and cytoplasm, consistent with its roles in transcription regulation and signaling.
• Slug is broadly expressed in adult human tissues, including the epidermis (basal layers), osteoblasts, and mesenchymal stem cells, with high expression in breast tumor cells.
• Associated with Waardenburg syndrome and deafness; involves in ubiquitin conjugation and protein degradation pathways.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein; the antibody is suitable for detecting endogenous Slug in WB and ELISA applications.
• Verify cross-reactivity with human and mouse samples, and consider validating in other relevant species if needed.
• For WB, expect a band at ~30 kDa; optimize blocking and detection conditions for your sample type.
• In ELISA, titrate the antibody to determine the optimal working concentration for your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined, one-stop access to premium IVD raw materials like the Anti-Slug antibody. Whether you need validated antibody pairs, bulk monoclonal antibodies, or ancillary reagents for EMT, cancer, and developmental biology research, our integrated supply chain ensures reliable sourcing from concept to clinic. Partner with CamelBio to accelerate your diagnostic or research projects with high-quality, traceable raw materials.