Anti-Slug Polyclonal Antibody for WB and ELISA - O43623

Core Product Specifications and Parameters

Biological Background: Slug Function and Localization

• Slug (Zinc finger protein SNAI2) is a transcription factor of the Snail family, containing zinc-finger DNA-binding domains, and acts as a transcriptional repressor.
• It plays a critical role in epithelial-mesenchymal transition (EMT) by repressing E-cadherin (CDH1) expression, promoting cell migration and invasion during development and cancer metastasis.
• Slug is essential for neural crest cell generation and migration, contributing to craniofacial development and pigment cell formation.
• In epidermal keratinocytes, Slug regulates integrin expression (ITGA3, ITGB1, ITGB4), influencing cell adhesion and proliferation.
• Slug subcellular localization is predominantly nuclear, where it binds to E-box sequences, but can also be detected in the cytoplasm.
• It is expressed in a broad range of adult tissues, including breast, prostate, ovary, and skin, with high levels in basal epidermal layers and osteoblasts.
• Slug is implicated in Waardenburg syndrome type 2D (an auditory-pigmentary disorder) and is classified as a developmental protein.
• The protein is subject to post-translational modifications such as ubiquitination and proteasomal degradation, which regulate its stability and activity.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa1-150) of human Slug; for Western blot, consider using nuclear extracts from Slug-expressing cell lines (e.g., MDA-MB-231, A375) to enhance detection.
• As Slug is a transcription factor with a molecular weight of ~30kDa, ensure appropriate gel percentage and transfer conditions; validate with positive and negative controls.
• For ELISA, coat with recombinant Slug protein or peptide; the antibody's cross-reactivity with mouse Slug allows use in mouse model studies.
• Given the antibody's polyclonal nature, lot-to-lot consistency may vary; it is advisable to titrate the antibody for optimal signal-to-noise ratio in your specific application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material sourcing, from validated antibody pairs to bulk ancillary reagents, supporting your research on transcription factors like Slug. Whether you need optimized antibodies for EMT and cancer studies or require rare target raw materials, our one-stop platform streamlines procurement from concept to clinic.