Anti-SLPI Rabbit Polyclonal Antibody for WB, ELISA - P03973

Core Product Specifications and Parameters

Biological Background: SLPI Function and Localization

• Antileukoproteinase (SLPI), also known as secretory leukocyte protease inhibitor, is an acid-stable serine protease inhibitor with high affinity for trypsin, chymotrypsin, elastase, and cathepsin G. Related references: PMID:10702419, PMID:2039600, PMID:2110563
• It modulates inflammatory and immune responses, down-regulating reactions to bacterial lipopolysaccharide (LPS) and regulating NF-kappa-B activation. Related references: PMID:10702419, PMID:24352879
• SLPI exhibits antimicrobial activity against mycobacteria and is required for normal resistance to M. tuberculosis infection, as well as defense against the intracellular parasite L. major.
• Together with ELANE, SLPI is required for normal differentiation and proliferation of bone marrow myeloid cells. Related references: PMID:24352879
• The protein contains two WAP four-disulfide core domains, characteristic of a family of protease inhibitors, and its structure is stabilized by conserved disulfide bonds.
• SLPI is a secreted protein detected in various body fluids and tissues, including blood plasma, airway sputum, parotid gland secretions, seminal plasma, and uterine cervix.
• It plays a critical role in wound healing, likely by preventing tissue damage through the limitation of excessive protease activity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 26–132 of human SLPI, corresponding to the mature protein after signal peptide cleavage; this recombinant fragment is expected to elicit antibodies recognizing the native folded protein.
• For Western blot analysis, consider using reducing conditions to fully dissociate potential disulfide-linked oligomers; a band around 14 kDa is anticipated for monomeric SLPI.
• Endogenous SLPI is secreted; when detecting in cellular models, supernatant concentration or epithelial cell lysates may improve signal, and appropriate positive controls (e.g., human airway sputum or seminal plasma) should be included.
• Validate the antibody in your specific sample matrix and application context, as performance can vary with tissue type and post-translational modifications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and life science researchers with comprehensive IVD raw material solutions, from validated antibody pairs and bulk ancillary reagents to rare target sourcing. This anti-SLPI polyclonal antibody addresses innate immunity and inflammation research, complementing our portfolio of optimized immunological reagents. Whether you require custom development or reliable supply chain continuity, CamelBio bridges your concept-to-clinic journey with quality raw materials and responsive technical support.