Anti-SLMAP Rabbit Polyclonal Antibody for WB, ELISA - Q14BN4

Core Product Specifications and Parameters

Biological Background: SLMAP Function and Localization

• SLMAP (Sarcolemmal membrane-associated protein), also referred to as SLAP, is encoded by the SLMAP gene (Gene ID: 7871) in humans.
• It is a key component of the striatin-interacting phosphatase and kinase (STRIPAK) core complex, forming the (SIKE1:SLMAP)STRIPAK complex, which dephosphorylates STK3 to inhibit Hippo signaling and regulate cell growth. Related references: PMID:29063833 PMID:30622739
• SLMAP may play a role in myoblast fusion, as suggested by sequence similarity.
• The protein exhibits a wide subcellular distribution, localizing to the sarcolemma, M-line and Z-line of sarcomeres, centrosome, and membranes of the endoplasmic reticulum and mitochondria.
• Alternative splicing generates multiple isoforms that contain coiled-coil and transmembrane domains, and the protein is regulated by phosphorylation.
• It is identified as a member of the reference proteome and has been detected in proteomics studies.

Experimental Guidance and Technical Tips

• For Western blot, use an appropriate total protein loading control to ensure accurate normalization; the expected molecular weight is approximately 95 kDa, but alternative isoforms may be present.
• Validate antibody specificity by performing knockdown or knockout experiments for SLMAP in the relevant cell or tissue system.
• Optimize ELISA conditions by titrating the coating antigen and testing a range of antibody dilutions; inclusion of appropriate positive and negative controls is recommended.
• Consider using freshly prepared samples and minimizing freeze-thaw cycles, as membrane-associated proteins may be prone to degradation.

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CamelBio is your dedicated one-stop sourcing bridge for IVD raw materials, supporting diagnostic developers and research laboratories from initial discovery to clinical application. Our extensive portfolio includes validated polyclonal antibodies like this anti-SLMAP antibody, along with matched antibody pairs, recombinant proteins, and bulk reagents tailored for cell signaling and cardiovascular research. Contact us to explore custom sourcing solutions for rare or specialized targets, ensuring your projects progress smoothly.