Anti-Slit2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O94813

Core Product Specifications and Parameters

Biological Background: Slit2 Function and Localization

• Slit2 is a secreted glycoprotein that functions as a molecular guidance cue in cellular migration, interacting with roundabout (Robo) receptors to mediate repulsive signals.
• During neural development, Slit2, along with Slit1, is essential for preventing inappropriate axonal crossing at the ventral midline of the forebrain and directing olfactory bulb projections.
• In the spinal cord, Slit2 modulates commissural axon responses to netrin and contributes to post-crossing repulsion at the floor plate.
• In the developing visual system, Slit2 acts as a repellent for retinal ganglion cell axons, collapsing growth cones and directing proper axonal pathfinding through the optic chiasm.
• The proteolytic N-product of Slit2 specifically repels olfactory bulb axons and induces branching of dorsal root ganglia (DRG) axons in vitro, while the C-product lacks these activities.
• Slit2 may also be implicated in regulating leukocyte migration, indicating functions beyond the nervous system.
• Subcellularly, Slit2 is localized to the extracellular space as a secreted protein, consistent with its role as a guidance cue.
• Tissue expression studies show prominent Slit2 expression in fetal lung and kidney, adult spinal cord, with weaker detection in adrenal gland, thyroid, and trachea.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 1400–1500 of human Slit2, located in the C-terminal region; verify sequence conservation across species for cross-reactivity assessment.
• For Western blot, expect a band near the theoretical molecular weight of 170 kDa; ensure adequate protein transfer and include appropriate molecular weight markers.
• In immunohistochemistry (IHC), consider performing antigen retrieval and validate the staining pattern using appropriate negative controls, such as knockout tissues or cell lines.
• For ELISA applications, the antibody may be used for capture or detection; optimize conditions using recombinant Slit2 protein or the immunizing peptide as reference standards.
• Given that Slit2 is secreted, analyze conditioned medium or use secretion inhibitors (e.g., brefeldin A) to capture intracellular protein pools when conducting cell-based assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material partner providing comprehensive support from concept to clinic. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for neurodevelopment and cell migration research. Whether you require consistent quality for routine assays or rare target sourcing for Slit2‑related projects, our one‑stop platform streamlines your procurement process.